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Pathogenesis of Experimental Encephalomyocarditis: A Histopathological, Immunohistochemical and Virological Study in Mice
Mice (n=20) aged 8 weeks were infected, either by oronasal inoculation or by contact, with one of two different myocardial strains of encephalomyocarditis virus (EMCV), namely, the Greek strain 424/90 and the Belgian strain B279/95. The animals were killed at 18–59 days post-infection (dpi), except...
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| Published in: | Journal of comparative pathology 2006-08, Vol.135 (2-3), p.142-145 |
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| container_end_page | 145 |
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| creator | Psalla, D. Psychas, V. Spyrou, V. Billinis, C. Papaioannou, N. Vlemmas, I. |
| description | Mice (n=20) aged 8 weeks were infected, either by oronasal inoculation or by contact, with one of two different myocardial strains of encephalomyocarditis virus (EMCV), namely, the Greek strain 424/90 and the Belgian strain B279/95. The animals were killed at 18–59 days post-infection (dpi), except for two mice that died at 6 and 32 dpi, and samples of brain, heart, pancreas, kidney, Peyer's patches, spleen, lung and thymus were processed for virological, histopathological and immunohistochemical examination. Apart from the two deaths, the experimental infection was inapparent, but virus was invariably recovered from faeces and several organs. The main histopathological lesions were focal interstitial pancreatitis, depletion of thymus and Peyer's patches, and interstitial pneumonia. Additionally, in the two mice that died, multifocal interstitial myocarditis was observed. EMCV antigen was detected in the cytoplasm of pancreatic acinar cells and in macrophages of the lung and the thymus. Antigen was also detected in the cytoplasm of cardiac muscle cells from three animals, including the two that died. The results support the role of mice, in addition to rats, as reservoir hosts in the epidemiology of EMCV infections on pig farms. |
| doi_str_mv | 10.1016/j.jcpa.2006.04.003 |
| format | article |
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The animals were killed at 18–59 days post-infection (dpi), except for two mice that died at 6 and 32 dpi, and samples of brain, heart, pancreas, kidney, Peyer's patches, spleen, lung and thymus were processed for virological, histopathological and immunohistochemical examination. Apart from the two deaths, the experimental infection was inapparent, but virus was invariably recovered from faeces and several organs. The main histopathological lesions were focal interstitial pancreatitis, depletion of thymus and Peyer's patches, and interstitial pneumonia. Additionally, in the two mice that died, multifocal interstitial myocarditis was observed. EMCV antigen was detected in the cytoplasm of pancreatic acinar cells and in macrophages of the lung and the thymus. Antigen was also detected in the cytoplasm of cardiac muscle cells from three animals, including the two that died. 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The results support the role of mice, in addition to rats, as reservoir hosts in the epidemiology of EMCV infections on pig farms.</description><subject>Animals</subject><subject>Antigens, Viral - metabolism</subject><subject>Cardiovirus Infections - etiology</subject><subject>Cardiovirus Infections - immunology</subject><subject>Cardiovirus Infections - transmission</subject><subject>Cardiovirus Infections - veterinary</subject><subject>disease course</subject><subject>disease reservoirs</subject><subject>Disease Reservoirs - veterinary</subject><subject>Disease Reservoirs - virology</subject><subject>EMCV</subject><subject>Encephalomyocarditis virus</subject><subject>Encephalomyocarditis virus - pathogenicity</subject><subject>Heart - virology</subject><subject>histopathology</subject><subject>laboratory animals</subject><subject>Lung - immunology</subject><subject>Lung - pathology</subject><subject>Lung - virology</subject><subject>Lung Diseases, Interstitial - etiology</subject><subject>Lung Diseases, Interstitial - immunology</subject><subject>Lung Diseases, Interstitial - pathology</subject><subject>lungs</subject><subject>macrophages</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>mouse</subject><subject>Myocarditis - immunology</subject><subject>Myocarditis - veterinary</subject><subject>Myocarditis - virology</subject><subject>Myocardium - immunology</subject><subject>Myocardium - pathology</subject><subject>pancreatitis</subject><subject>Pancreatitis - etiology</subject><subject>Pancreatitis - immunology</subject><subject>Pancreatitis - pathology</subject><subject>Peyer's patches</subject><subject>Peyer's Patches - immunology</subject><subject>Peyer's Patches - pathology</subject><subject>Peyer's Patches - virology</subject><subject>strain differences</subject><subject>Swine</subject><subject>Swine Diseases - virology</subject><subject>thymus gland</subject><subject>Thymus Gland - immunology</subject><subject>Thymus Gland - pathology</subject><subject>Thymus Gland - virology</subject><subject>tissue distribution</subject><subject>viral infection</subject><issn>0021-9975</issn><issn>1532-3129</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNp9kF2L1DAUhoMo7rj6B7zQXHll60mafok3yzK6CysK63ob0uRkJkPbdJNWnH9vyox4ZwgEznnel_AQ8ppBzoBVHw75QU8q5wBVDiIHKJ6QDSsLnhWMt0_JBoCzrG3r8oK8iPEAAE1T8ufkglVtyYsaNuT4Xc17v8MRo4vUW7r9PWFwA46z6ul21DjtVe-Ho9cqGDe7-JFe0RsXZz-tyd7vnFb9e3o7DMvo9-tC73FYh1SNhv504S9E7-fFHKkb6Ven8SV5ZlUf8dX5vSQPn7c_rm-yu29fbq-v7jIthJizGlTHOy3Kjtu6UG3TibJUaA1XrWm16kAVoJMPVTQoKltbWyE0ljXadFw0xSV5d-qdgn9cMM5ycFFj36sR_RJl1aTDQCSQn0AdfIwBrZySCBWOkoFchcuDXIXLVbgEIZPwFHpzbl-6Ac2_yNlwAt6eAKu8VLvgony458AKYIy16Sbi04nAZOGXwyCjdpjMGxdQz9J4978f_AFT_Z4C</recordid><startdate>20060801</startdate><enddate>20060801</enddate><creator>Psalla, D.</creator><creator>Psychas, V.</creator><creator>Spyrou, V.</creator><creator>Billinis, C.</creator><creator>Papaioannou, N.</creator><creator>Vlemmas, I.</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060801</creationdate><title>Pathogenesis of Experimental Encephalomyocarditis: A Histopathological, Immunohistochemical and Virological Study in Mice</title><author>Psalla, D. ; Psychas, V. ; Spyrou, V. ; Billinis, C. ; Papaioannou, N. ; Vlemmas, I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c444t-70ab2bc45b2f73a98b455aefd2a9d9cab0a30c101a38e46f7ff6e08f18cdb2483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Antigens, Viral - metabolism</topic><topic>Cardiovirus Infections - etiology</topic><topic>Cardiovirus Infections - immunology</topic><topic>Cardiovirus Infections - transmission</topic><topic>Cardiovirus Infections - veterinary</topic><topic>disease course</topic><topic>disease reservoirs</topic><topic>Disease Reservoirs - veterinary</topic><topic>Disease Reservoirs - virology</topic><topic>EMCV</topic><topic>Encephalomyocarditis virus</topic><topic>Encephalomyocarditis virus - pathogenicity</topic><topic>Heart - virology</topic><topic>histopathology</topic><topic>laboratory animals</topic><topic>Lung - immunology</topic><topic>Lung - pathology</topic><topic>Lung - virology</topic><topic>Lung Diseases, Interstitial - etiology</topic><topic>Lung Diseases, Interstitial - immunology</topic><topic>Lung Diseases, Interstitial - pathology</topic><topic>lungs</topic><topic>macrophages</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>mouse</topic><topic>Myocarditis - immunology</topic><topic>Myocarditis - veterinary</topic><topic>Myocarditis - virology</topic><topic>Myocardium - immunology</topic><topic>Myocardium - pathology</topic><topic>pancreatitis</topic><topic>Pancreatitis - etiology</topic><topic>Pancreatitis - immunology</topic><topic>Pancreatitis - pathology</topic><topic>Peyer's patches</topic><topic>Peyer's Patches - immunology</topic><topic>Peyer's Patches - pathology</topic><topic>Peyer's Patches - virology</topic><topic>strain differences</topic><topic>Swine</topic><topic>Swine Diseases - virology</topic><topic>thymus gland</topic><topic>Thymus Gland - immunology</topic><topic>Thymus Gland - pathology</topic><topic>Thymus Gland - virology</topic><topic>tissue distribution</topic><topic>viral infection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Psalla, D.</creatorcontrib><creatorcontrib>Psychas, V.</creatorcontrib><creatorcontrib>Spyrou, V.</creatorcontrib><creatorcontrib>Billinis, C.</creatorcontrib><creatorcontrib>Papaioannou, N.</creatorcontrib><creatorcontrib>Vlemmas, I.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of comparative pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Psalla, D.</au><au>Psychas, V.</au><au>Spyrou, V.</au><au>Billinis, C.</au><au>Papaioannou, N.</au><au>Vlemmas, I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pathogenesis of Experimental Encephalomyocarditis: A Histopathological, Immunohistochemical and Virological Study in Mice</atitle><jtitle>Journal of comparative pathology</jtitle><addtitle>J Comp Pathol</addtitle><date>2006-08-01</date><risdate>2006</risdate><volume>135</volume><issue>2-3</issue><spage>142</spage><epage>145</epage><pages>142-145</pages><issn>0021-9975</issn><eissn>1532-3129</eissn><abstract>Mice (n=20) aged 8 weeks were infected, either by oronasal inoculation or by contact, with one of two different myocardial strains of encephalomyocarditis virus (EMCV), namely, the Greek strain 424/90 and the Belgian strain B279/95. The animals were killed at 18–59 days post-infection (dpi), except for two mice that died at 6 and 32 dpi, and samples of brain, heart, pancreas, kidney, Peyer's patches, spleen, lung and thymus were processed for virological, histopathological and immunohistochemical examination. Apart from the two deaths, the experimental infection was inapparent, but virus was invariably recovered from faeces and several organs. The main histopathological lesions were focal interstitial pancreatitis, depletion of thymus and Peyer's patches, and interstitial pneumonia. Additionally, in the two mice that died, multifocal interstitial myocarditis was observed. EMCV antigen was detected in the cytoplasm of pancreatic acinar cells and in macrophages of the lung and the thymus. Antigen was also detected in the cytoplasm of cardiac muscle cells from three animals, including the two that died. The results support the role of mice, in addition to rats, as reservoir hosts in the epidemiology of EMCV infections on pig farms.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>16952370</pmid><doi>10.1016/j.jcpa.2006.04.003</doi><tpages>4</tpages></addata></record> |
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| ispartof | Journal of comparative pathology, 2006-08, Vol.135 (2-3), p.142-145 |
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| subjects | Animals Antigens, Viral - metabolism Cardiovirus Infections - etiology Cardiovirus Infections - immunology Cardiovirus Infections - transmission Cardiovirus Infections - veterinary disease course disease reservoirs Disease Reservoirs - veterinary Disease Reservoirs - virology EMCV Encephalomyocarditis virus Encephalomyocarditis virus - pathogenicity Heart - virology histopathology laboratory animals Lung - immunology Lung - pathology Lung - virology Lung Diseases, Interstitial - etiology Lung Diseases, Interstitial - immunology Lung Diseases, Interstitial - pathology lungs macrophages Mice Mice, Inbred BALB C mouse Myocarditis - immunology Myocarditis - veterinary Myocarditis - virology Myocardium - immunology Myocardium - pathology pancreatitis Pancreatitis - etiology Pancreatitis - immunology Pancreatitis - pathology Peyer's patches Peyer's Patches - immunology Peyer's Patches - pathology Peyer's Patches - virology strain differences Swine Swine Diseases - virology thymus gland Thymus Gland - immunology Thymus Gland - pathology Thymus Gland - virology tissue distribution viral infection |
| title | Pathogenesis of Experimental Encephalomyocarditis: A Histopathological, Immunohistochemical and Virological Study in Mice |
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