Pathogenesis of Experimental Encephalomyocarditis: A Histopathological, Immunohistochemical and Virological Study in Mice

Mice (n=20) aged 8 weeks were infected, either by oronasal inoculation or by contact, with one of two different myocardial strains of encephalomyocarditis virus (EMCV), namely, the Greek strain 424/90 and the Belgian strain B279/95. The animals were killed at 18–59 days post-infection (dpi), except...

Full description

Saved in:
Bibliographic Details
Published in:Journal of comparative pathology 2006-08, Vol.135 (2-3), p.142-145
Main Authors: Psalla, D., Psychas, V., Spyrou, V., Billinis, C., Papaioannou, N., Vlemmas, I.
Format: Article
Language:English
Subjects:
Animals
Antigens, Viral - metabolism
Cardiovirus Infections - etiology
Cardiovirus Infections - immunology
Cardiovirus Infections - transmission
Cardiovirus Infections - veterinary
disease course
disease reservoirs
Disease Reservoirs - veterinary
Disease Reservoirs - virology
EMCV
Encephalomyocarditis virus
Encephalomyocarditis virus - pathogenicity
Heart - virology
histopathology
laboratory animals
Lung - immunology
Lung - pathology
Lung - virology
Lung Diseases, Interstitial - etiology
Lung Diseases, Interstitial - immunology
Lung Diseases, Interstitial - pathology
lungs
macrophages
Mice
Mice, Inbred BALB C
mouse
Myocarditis - immunology
Myocarditis - veterinary
Myocarditis - virology
Myocardium - immunology
Myocardium - pathology
pancreatitis
Pancreatitis - etiology
Pancreatitis - immunology
Pancreatitis - pathology
Peyer's patches
Peyer's Patches - immunology
Peyer's Patches - pathology
Peyer's Patches - virology
strain differences
Swine
Swine Diseases - virology
thymus gland
Thymus Gland - immunology
Thymus Gland - pathology
Thymus Gland - virology
tissue distribution
viral infection
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Mice (n=20) aged 8 weeks were infected, either by oronasal inoculation or by contact, with one of two different myocardial strains of encephalomyocarditis virus (EMCV), namely, the Greek strain 424/90 and the Belgian strain B279/95. The animals were killed at 18–59 days post-infection (dpi), except for two mice that died at 6 and 32 dpi, and samples of brain, heart, pancreas, kidney, Peyer's patches, spleen, lung and thymus were processed for virological, histopathological and immunohistochemical examination. Apart from the two deaths, the experimental infection was inapparent, but virus was invariably recovered from faeces and several organs. The main histopathological lesions were focal interstitial pancreatitis, depletion of thymus and Peyer's patches, and interstitial pneumonia. Additionally, in the two mice that died, multifocal interstitial myocarditis was observed. EMCV antigen was detected in the cytoplasm of pancreatic acinar cells and in macrophages of the lung and the thymus. Antigen was also detected in the cytoplasm of cardiac muscle cells from three animals, including the two that died. The results support the role of mice, in addition to rats, as reservoir hosts in the epidemiology of EMCV infections on pig farms.
ISSN:0021-9975
1532-3129
DOI:10.1016/j.jcpa.2006.04.003