Reduced osteoblastic population and defective mineralization in osteopetrotic (op/op) mice

Osteopetrotic (op/op) mice fail to exhibit bone remodeling because of a defective osteoclast formation due to a lack of macrophage colony-stimulating factor. In this study, we investigated the femora of op/op mice to clarify whether the osteoblastic population and bone mineralization are involved in...

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Bibliographic Details
Published in:Micron (Oxford, England : 1993) England : 1993), 2005-01, Vol.36 (7), p.688-695
Main Authors: Sakagami, Naoko, Amizuka, Norio, Li, Minqi, Takeuchi, Kiichi, Hoshino, Masaaki, Nakamura, Midori, Nozawa-Inoue, Kayoko, Udagawa, Nobuyuki, Maeda, Takeyasu
Format: Article
Language:English
Subjects:
Alkaline Phosphatase - analysis
Animals
Biomechanical Phenomena
Bone Matrix - pathology
Bone Matrix - physiopathology
Bone Matrix - ultrastructure
Bone Remodeling - physiology
Calcification, Physiologic
Connective Tissue - pathology
Connective Tissue - physiopathology
Connective Tissue - ultrastructure
Diaphyses - pathology
Diaphyses - physiopathology
Diaphyses - ultrastructure
Disease Models, Animal
Femur
Growth Plate - enzymology
Growth Plate - pathology
Growth Plate - physiopathology
Growth Plate - ultrastructure
Haversian System - pathology
Haversian System - physiopathology
Haversian System - ultrastructure
Immunohistochemistry
Mice
Mice, Mutant Strains
Microscopy, Electron, Transmission
Mineralization
Op/op mouse
Osteoblast
Osteoblasts - pathology
Osteoblasts - physiology
Osteoblasts - ultrastructure
Osteoclast
Osteoclasts - pathology
Osteoclasts - physiology
Osteoclasts - ultrastructure
Osteopetrosis
Osteopetrosis - genetics
Osteopetrosis - pathology
Osteopetrosis - physiopathology
Tibia
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Summary:Osteopetrotic (op/op) mice fail to exhibit bone remodeling because of a defective osteoclast formation due to a lack of macrophage colony-stimulating factor. In this study, we investigated the femora of op/op mice to clarify whether the osteoblastic population and bone mineralization are involved in osteoclasts or their bone resorption. The op/op mice extended the meshwork of trabecular bones from the chondro-osseous junction to the diaphyseal region. In the femoral metaphyses of op/op mice, intense alkaline phosphatase (ALPase)-positive osteoblasts were observed on the metaphyseal bone in close proximity to the erosion zone of the growth plates. Von Kossa's staining revealed scattered mineralized nodules and a fine meshwork of mineralized bone matrices while the wild-type littermates developed well-mineralized trabeculae parallel to the longitudinal axis. In contrast to the metaphysis, some op/op diaphyses showed flattened osteoblasts with weak ALPase-positivity, and the other diaphyses displayed bone surfaces without a covering by osteoblasts. It is likely, therefore, that the osteoblastic population and activity were lessened in the op/op diaphyses. Despite the osteopetrotic model, von Kossa's staining demonstrated patchy unmineralized areas in the op/op diaphyses, indicating that a lower population and/or the activity of osteoblasts resulted in defective mineralization in the bone. Transmission electron microscopy disclosed few osteoblasts on the diaphyseal bones, and instead, bone marrow cells and vascular endothelial cells were often attached to the unmineralized bone. Osteocytes were embedded in the unmineralized bone matrix. Thus, osteoclasts appear to be involved in the osteoblastic population and activity as well as subsequent bone mineralization.
ISSN:0968-4328
1878-4291
DOI:10.1016/j.micron.2005.06.008