Microcyn : a novel super-oxidized water with neutral pH and disinfectant activity

A new super-oxidized water (SOW) product, Microcyn, was tested for in vitro antimicrobial and antiviral activities. The effectiveness of this neutral-pH SOW at killing Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi and Candida albicans in pure culture was evaluated...

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Bibliographic Details
Published in:The Journal of hospital infection 2005-12, Vol.61 (4), p.291-299
Main Authors: LANDA-SOLIS, C, GONZALEZ-ESPINOSA, D, GUZMAN-SORIANO, B, SNYDER, M, REYES-TERAN, G, TORRES, K, GUTIERREZ, A. A
Format: Article
Language:English
Subjects:
Adenoviridae - drug effects
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antiseptics
Bacillus
Bacteria - drug effects
Biological and medical sciences
Candida albicans
Candida albicans - drug effects
Cell Line
Colony Count, Microbial
Cytopathogenic Effect, Viral
Disinfectants - pharmacology
Epidemiology. Vaccinations
Escherichia
Flow Cytometry
Fluorescence
General aspects
Green Fluorescent Proteins - analysis
Green Fluorescent Proteins - genetics
HIV Core Protein p24 - analysis
HIV-1 - drug effects
Human immunodeficiency virus 1
Humans
Hydrogen Peroxide - pharmacology
Hydrogen-Ion Concentration
Infectious diseases
Medical sciences
Pharmacology. Drug treatments
Salmonella typhi
Spores, Bacterial - drug effects
Staphylococcus aureus
Viral Load
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Summary:A new super-oxidized water (SOW) product, Microcyn, was tested for in vitro antimicrobial and antiviral activities. The effectiveness of this neutral-pH SOW at killing Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi and Candida albicans in pure culture was evaluated. One millilitre (approximately 10(8)colony-forming units/mL) of each micro-organism was subjected to 9 mL Microcyn or sterile water at room temperature for 30s. Under these conditions, a log(10) reduction factor of 8 in the level of all pathogens occurred in the treatment samples. In addition, results of tests with three batches of Microcyn exposed to Bacillus atrophaeus spores for 5 min demonstrated complete inactivation of the spores within 2-3 min (log(10) reduction factor >4). The effectiveness of Microcyn in reducing human immunodeficiency virus-1 (HIV-1) on hard surfaces (glass) was also evaluated in compliance with Environmental Protection Agency requirements for virucidal claims. After exposure of the tested surfaces to Microcyn for 5 min without agitation, there was a log(10) reduction factor >3 in the viral load as measured by both cytopathic effect and antigen p24 of HIV-1 production in MT-2 cultures. Microcyn activity against adenoviral vector type 5 was also analysed under simulated laboratory in-use conditions with viral suspensions. In order to increase the sensitivity of the test, the fluorescent light emitted by AdGFP-infected cells was measured with the use of a flow cytometer. A log(10) reduction factor >3 in the viral load was achieved after a 5-min exposure to Microcyn under these strict conditions. These results show that Microcyn exerts a wide antimicrobial spectrum with major advantages over acidic SOWs, including neutral pH, lower free active chlorine (51-85 ppm) and long shelf life (1 year).
ISSN:0195-6701
1532-2939
DOI:10.1016/j.jhin.2005.04.021