Evaluation of a 2% chlorhexidine gluconate in 70% isopropyl alcohol skin disinfectant

The efficacy of a new skin disinfectant, 2% (w/v) chlorhexidine gluconate (CHG) in 70% (v/v) isopropyl alcohol (IPA) (ChloraPrep ®), was compared with five commonly used skin disinfectants against Staphylococcus epidermidis RP62A in the presence or absence of protein, utilizing quantitative time-kil...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of hospital infection 2005-12, Vol.61 (4), p.287-290
Main Authors: Adams, D., Quayum, M., Worthington, T., Lambert, P., Elliott, T.
Format: Article
Language:English
Subjects:
2-Propanol - pharmacology
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antiseptics
Biofilms - drug effects
Biological and medical sciences
ChloraPrep
Chlorhexidine
Chlorhexidine - analogs & derivatives
Chlorhexidine - pharmacology
Colony Count, Microbial
Communicable Disease Control - methods
Disinfectant
Disinfectants - chemistry
Disinfectants - pharmacology
Humans
Infectious diseases
Isopropanol
Medical sciences
Pharmacology. Drug treatments
Povidone iodine
Skin - microbiology
Skin antisepsis
Staphylococcus epidermidis
Staphylococcus epidermidis - drug effects
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The efficacy of a new skin disinfectant, 2% (w/v) chlorhexidine gluconate (CHG) in 70% (v/v) isopropyl alcohol (IPA) (ChloraPrep ®), was compared with five commonly used skin disinfectants against Staphylococcus epidermidis RP62A in the presence or absence of protein, utilizing quantitative time-kill suspension and carrier tests. All six disinfectants [70% (v/v) IPA, 0.5% (w/v) aqueous CHG, 2% (w/v) aqueous CHG, 0.5% (w/v) CHG in 70% (v/v) IPA and 10% (w/v) aqueous povidone iodine (PI)] achieved a log 10 reduction factor of 5, in colony-forming units/mL, in a suspension test (exposure time 30 s) in the presence and absence of 10% human serum. Subsequent challenges of S. epidermidis RP62A in a biofilm (with and without human serum) demonstrated reduced bactericidal activity. Overall, the most effective skin disinfectants tested against S. epidermidis RP62A were 2% (w/v) CHG in 70% IPA and 10% (w/v) PI. These results suggest that enhanced skin antisepsis may be achieved with 2% (w/v) CHG in 70% (v/v) IPA compared with the three commonly used CHG preparations [0.5% (w/v) aqueous CHG, 2% (w/v) aqueous CHG and 0.5% (w/v) CHG in 70% (v/v) IPA].
ISSN:0195-6701
1532-2939
DOI:10.1016/j.jhin.2005.05.015