Metabolite profile of sibutramine in human urine: a liquid chromatography-electrospray ionization mass spectrometric study

We present a detailed experimental approach to detection and subsequent structural characterization of unknown metabolites of sibutramine, using liquid chromatography‐mass spectrometric techniques. The full‐, precursor ion, and constant neutral loss scan modes of a triple quadrupole mass spectromete...

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Bibliographic Details
Published in:Journal of mass spectrometry. 2006-09, Vol.41 (9), p.1171-1178
Main Authors: Link, Marek, Hakala, Kati S., Wsól, Vladimír, Kostiainen, Risto, Ketola, Raimo A.
Format: Article
Language:English
Subjects:
Adult
Antidepressive Agents - urine
Biological and medical sciences
Biotransformation
carbamoyl glucuronides
Chromatography, Liquid
Cyclobutanes - urine
drug metabolism
Ethanol - chemistry
Female
General pharmacology
Glucuronides - urine
Humans
Hydroxylation
Indicators and Reagents
Medical sciences
metabolite identification
Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions
Pharmacology. Drug treatments
Reference Standards
sibutramine
Solvents
Spectrometry, Mass, Electrospray Ionization
tandem mass spectrometry
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Summary:We present a detailed experimental approach to detection and subsequent structural characterization of unknown metabolites of sibutramine, using liquid chromatography‐mass spectrometric techniques. The full‐, precursor ion, and constant neutral loss scan modes of a triple quadrupole mass spectrometer were used for screening sibutramine metabolites in human urine. The structural assessment of unknown metabolites was based on MSn ion trap mass spectrometric analysis and comparison of MSn spectra between the standards and compounds detected. Two phase‐I (M1 and M2) and eight phase‐II (M3‐M6) metabolites of sibutramine were found in human urine. Metabolites M1 and M2, which were found as minor metabolites, originated from N‐demethylation of sibutramine. Carbamoyl glucuronides formed from metabolites M1, M2, and their hydroxylated analogs were the main metabolites of sibutramine and were characterized by tandem mass spectrometric analysis and by the chemical modification of their structure. We demonstrate the usefulness of the chemical derivatization approach for estimation of the site of glucuronidation and propose the formation of hydroxylated regioisomers of metabolites M4 and M6. Copyright © 2006 John Wiley & Sons, Ltd.
ISSN:1076-5174
1096-9888
DOI:10.1002/jms.1082