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Sedimentation field flow fractionation monitoring of bimodal wheat starch amylolysis
Enzymatic starch granule hydrolysis is one of the most important reactions in many industrial processes. In this study, we investigated the capacity of sedimentation field flow fractionation (SdFFF) to monitor the amylolysis of a bimodal starch population: native wheat starch. Results demonstrated a...
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Published in: | Journal of Chromatography A 2006-10, Vol.1129 (2), p.247-254 |
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container_title | Journal of Chromatography A |
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creator | Salesse, C. Battu, S. Begaud-Grimaud, G. Cledat, D. Cook-Moreau, J. Cardot, P.J.P. |
description | Enzymatic starch granule hydrolysis is one of the most important reactions in many industrial processes. In this study, we investigated the capacity of sedimentation field flow fractionation (SdFFF) to monitor the amylolysis of a bimodal starch population: native wheat starch. Results demonstrated a correlation between fractogram changes and enzymatic hydrolysis. Furthermore, SdFFF was used to sort sub-populations which enhanced the study of granule size distribution changes occurring during amylolysis. These results show the interest in coupling SdFFF with particle size measurement methods to study complex starch size/density modifications associated to hydrolysis. These results suggested different applications such as the association of SdFFF with structural investigations to better understand the specific mechanisms of amylolysis or starch granule structure. |
doi_str_mv | 10.1016/j.chroma.2006.06.104 |
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In this study, we investigated the capacity of sedimentation field flow fractionation (SdFFF) to monitor the amylolysis of a bimodal starch population: native wheat starch. Results demonstrated a correlation between fractogram changes and enzymatic hydrolysis. Furthermore, SdFFF was used to sort sub-populations which enhanced the study of granule size distribution changes occurring during amylolysis. These results show the interest in coupling SdFFF with particle size measurement methods to study complex starch size/density modifications associated to hydrolysis. 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These results suggested different applications such as the association of SdFFF with structural investigations to better understand the specific mechanisms of amylolysis or starch granule structure.</description><subject>Amylase</subject><subject>Amylases - metabolism</subject><subject>Amylolysis</subject><subject>Biological and medical sciences</subject><subject>Food industries</subject><subject>Fractionation, Field Flow - instrumentation</subject><subject>Fractionation, Field Flow - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrolysis</subject><subject>Native starch</subject><subject>Particle Size</subject><subject>Reproducibility of Results</subject><subject>Sedimentation field flow fractionation</subject><subject>Starch - analysis</subject><subject>Starch - metabolism</subject><subject>Starch and starchy product industries</subject><subject>Triticum - chemistry</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNp9kE1rHDEMhn1oSdKk_6CUubS33cgzs_64FEpom0AghyRno7HlrhfPOLVnG_bf18ss5FYQCKTnFeJh7BOHNQcurndru81pxHULINa1OPTv2AVAy1dayO6cfShlB8AlyPaMnXOhJHC9uWBPj-TCSNOMc0hT4wNF1_iYXhuf0R5ny2JMU5hTDtPvJvlmCGNyGJvXLeHclBmz3TY4HmKKhxLKFXvvMRb6eOqX7Pnnj6eb29X9w6-7m-_3K9tpmFfCac8dWfKaNgKlbgEH1H1rtVM9gsChBdH3xOVm4JZIKdn5QYmOJGrrukv2dbn7ktOfPZXZjKFYihEnSvtihFICetVXsF9Am1Mpmbx5yWHEfDAczNGg2ZnFoDkaNLWqwRr7fLq_H0Zyb6GTvgp8OQFYLMaqbLKhvHGKt9BKqNy3haNq42-gbIoNNNnqPpOdjUvh_5_8A7oclPs</recordid><startdate>20061006</startdate><enddate>20061006</enddate><creator>Salesse, C.</creator><creator>Battu, S.</creator><creator>Begaud-Grimaud, G.</creator><creator>Cledat, D.</creator><creator>Cook-Moreau, J.</creator><creator>Cardot, P.J.P.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20061006</creationdate><title>Sedimentation field flow fractionation monitoring of bimodal wheat starch amylolysis</title><author>Salesse, C. ; Battu, S. ; Begaud-Grimaud, G. ; Cledat, D. ; Cook-Moreau, J. ; Cardot, P.J.P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-6d9f1decef9e56a7920aba942c9d84a06ab20644e175b1cee8873fb863e7a9cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Amylase</topic><topic>Amylases - metabolism</topic><topic>Amylolysis</topic><topic>Biological and medical sciences</topic><topic>Food industries</topic><topic>Fractionation, Field Flow - instrumentation</topic><topic>Fractionation, Field Flow - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrolysis</topic><topic>Native starch</topic><topic>Particle Size</topic><topic>Reproducibility of Results</topic><topic>Sedimentation field flow fractionation</topic><topic>Starch - analysis</topic><topic>Starch - metabolism</topic><topic>Starch and starchy product industries</topic><topic>Triticum - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Salesse, C.</creatorcontrib><creatorcontrib>Battu, S.</creatorcontrib><creatorcontrib>Begaud-Grimaud, G.</creatorcontrib><creatorcontrib>Cledat, D.</creatorcontrib><creatorcontrib>Cook-Moreau, J.</creatorcontrib><creatorcontrib>Cardot, P.J.P.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Salesse, C.</au><au>Battu, S.</au><au>Begaud-Grimaud, G.</au><au>Cledat, D.</au><au>Cook-Moreau, J.</au><au>Cardot, P.J.P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sedimentation field flow fractionation monitoring of bimodal wheat starch amylolysis</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>2006-10-06</date><risdate>2006</risdate><volume>1129</volume><issue>2</issue><spage>247</spage><epage>254</epage><pages>247-254</pages><issn>0021-9673</issn><coden>JOCRAM</coden><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Enzymatic starch granule hydrolysis is one of the most important reactions in many industrial processes. 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subjects | Amylase Amylases - metabolism Amylolysis Biological and medical sciences Food industries Fractionation, Field Flow - instrumentation Fractionation, Field Flow - methods Fundamental and applied biological sciences. Psychology Hydrolysis Native starch Particle Size Reproducibility of Results Sedimentation field flow fractionation Starch - analysis Starch - metabolism Starch and starchy product industries Triticum - chemistry |
title | Sedimentation field flow fractionation monitoring of bimodal wheat starch amylolysis |
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