Sedimentation field flow fractionation monitoring of bimodal wheat starch amylolysis

Enzymatic starch granule hydrolysis is one of the most important reactions in many industrial processes. In this study, we investigated the capacity of sedimentation field flow fractionation (SdFFF) to monitor the amylolysis of a bimodal starch population: native wheat starch. Results demonstrated a...

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Bibliographic Details
Published in:Journal of Chromatography A 2006-10, Vol.1129 (2), p.247-254
Main Authors: Salesse, C., Battu, S., Begaud-Grimaud, G., Cledat, D., Cook-Moreau, J., Cardot, P.J.P.
Format: Article
Language:English
Subjects:
Amylase
Amylases - metabolism
Amylolysis
Biological and medical sciences
Food industries
Fractionation, Field Flow - instrumentation
Fractionation, Field Flow - methods
Fundamental and applied biological sciences. Psychology
Hydrolysis
Native starch
Particle Size
Reproducibility of Results
Sedimentation field flow fractionation
Starch - analysis
Starch - metabolism
Starch and starchy product industries
Triticum - chemistry
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Summary:Enzymatic starch granule hydrolysis is one of the most important reactions in many industrial processes. In this study, we investigated the capacity of sedimentation field flow fractionation (SdFFF) to monitor the amylolysis of a bimodal starch population: native wheat starch. Results demonstrated a correlation between fractogram changes and enzymatic hydrolysis. Furthermore, SdFFF was used to sort sub-populations which enhanced the study of granule size distribution changes occurring during amylolysis. These results show the interest in coupling SdFFF with particle size measurement methods to study complex starch size/density modifications associated to hydrolysis. These results suggested different applications such as the association of SdFFF with structural investigations to better understand the specific mechanisms of amylolysis or starch granule structure.
ISSN:0021-9673
DOI:10.1016/j.chroma.2006.06.104