Fast analysis in liquid chromatography using small particle size and high pressure

In order to enhance chromatographic performances in terms of efficiency and rapidity, LC has recently evolved in the development of short columns packed with small particles (sub‐2 μm) working at high pressures (>400 bar). This approach has been described 30 years ago according to the fundamental...

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Bibliographic Details
Published in:Journal of separation science 2006-08, Vol.29 (12), p.1836-1848
Main Authors: Nguyen, Dao T.-T., Guillarme, Davy, Rudaz, Serge, Veuthey, Jean-Luc
Format: Article
Language:English
Subjects:
Analysis
Analytical chemistry
Biological and medical sciences
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, Liquid - instrumentation
Chromatography, Liquid - methods
Equipment Design
Exact sciences and technology
Fast LC
General pharmacology
Medical sciences
Other chromatographic methods
Particle Size
Pharmacology. Drug treatments
Pressure
Sensitivity and Specificity
Small particles
Sub-2 μm particle
Surface Properties
Time Factors
Ultra-high pressure
UPLC
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Summary:In order to enhance chromatographic performances in terms of efficiency and rapidity, LC has recently evolved in the development of short columns packed with small particles (sub‐2 μm) working at high pressures (>400 bar). This approach has been described 30 years ago according to the fundamental chromatographic equations. However, systems and columns compatible with such high pressures have been introduced in the market in 2004 only. Advantages of small particles working at high pressure will be discussed in terms of sensitivity, efficiency, resolution, and analysis time. Potential problems encountered with high pressure in terms of frictional heating and solvent compressibility will also be discussed even if systems working at a maximum pressure of 1000 bar are not influenced by these parameters and give reliable and reproducible results. Several applications will highlight the potential and interest of this new technology.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.200600189