Morphological alterations of Metarhizium anisopliae during penetration of Boophilus microplus ticks

Chronological histological alterations of Metarhizium anisopliae during interaction with the cattle tick Boophilus microplus were investigated by light and scanning electron microscopy. M. anisopliae invades B. microplus by a process which involves adhesion of conidia to the cuticle, conidia germina...

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Bibliographic Details
Published in:Experimental & applied acarology 2005-12, Vol.37 (3-4), p.231-244
Main Authors: Arruda, W, Lubeck, I, Schrank, A, Vainstein, M.H
Format: Article
Language:English
Subjects:
adhesion
animal cuticle
Animals
Biological and medical sciences
biological control agents
Boophilus microplus
Cattle
Cattle Diseases - parasitology
Cattle Diseases - prevention & control
cuticular penetration
entomopathogenic fungi
Fundamental and applied biological sciences. Psychology
fungal anatomy
Hyphae - growth & development
Hyphae - ultrastructure
Hypocreales - growth & development
Hypocreales - ultrastructure
infection
Ixodidae
Ixodidae - microbiology
Ixodidae - ultrastructure
Medically important nuisances and vectors, pests of stored products and materials: population survey and control
Metarhizium anisopliae
Microscopy, Electron, Scanning - methods
pathogenesis
Pest Control, Biological
tick control
Tick Infestations - parasitology
Tick Infestations - prevention & control
Tick Infestations - veterinary
Vectors. Intermediate hosts
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Summary:Chronological histological alterations of Metarhizium anisopliae during interaction with the cattle tick Boophilus microplus were investigated by light and scanning electron microscopy. M. anisopliae invades B. microplus by a process which involves adhesion of conidia to the cuticle, conidia germination, formation of appressoria and penetration through the cuticle. Twenty-four hours post-infection conidia are adhered and germination starts on the surface of the tick. At this time, the conidia differentiate to form appressoria exerting mechanical pressure and trigger hydrolytic enzyme secretion leading to penetration. Massive penetration is observed 72 h post-inoculation, and after 96 h, the hyphae start to emerge from the cuticle surface to form conidia. The intense invasion of adjacent tissues by hyphae was observed by light microscopy, confirming the ability of M. anisopliae to produce significant morphological alterations in the cuticle, and its infective effectiveness in B. microplus.
ISSN:0168-8162
1572-9702
DOI:10.1007/s10493-005-3818-6