KCTD11 expression in medulloblastoma is lower than in adult cerebellum and higher than in neural stem cells

Medulloblastoma (MB) is the most common malignant brain tumor of childhood, and the most frequent associated genetic alteration is loss of heterozygosity on chromosome region 7p13. Two genes mapping to this region, KCTD11 (alias REN) and HIC1, have been proposed as involved in MB pathogenesis. We us...

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Published in:Cancer genetics and cytogenetics 2006-10, Vol.170 (1), p.24-28
Main Authors: Zawlik, Izabela, Zakrzewska, Magdalena, Witusik, Monika, Golanska, Ewa, Kulczycka-Wojdala, Dominika, Szybka, Malgorzata, Piaskowski, Sylwester, Wozniak, Krystyna, Zakrzewski, Krzysztof, Papierz, Wielislaw, Liberski, Pawel P., Rieske, Piotr
Format: Article
Language:English
Subjects:
Adolescent
Base Sequence
Cerebellum - metabolism
Child
Child, Preschool
DNA Methylation
DNA Primers
DNA-Binding Proteins - genetics
Female
Humans
Kruppel-Like Transcription Factors
Loss of Heterozygosity
Male
Medulloblastoma - genetics
Nervous System - cytology
Nervous System - metabolism
Polymerase Chain Reaction
Potassium Channels - genetics
Stem Cells - metabolism
Transcription Factors - genetics
Transcription, Genetic
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Summary:Medulloblastoma (MB) is the most common malignant brain tumor of childhood, and the most frequent associated genetic alteration is loss of heterozygosity on chromosome region 7p13. Two genes mapping to this region, KCTD11 (alias REN) and HIC1, have been proposed as involved in MB pathogenesis. We used real-time polymerase chain reaction in 20 tissue samples of primary MB to examine the transcriptional level of the two genes, with reference to two types of controls: adult cerebellum and fetal neural stem cells. A significant reduction of KCTD11 expression relative to adult normal cerebellum was detected in 14 of 20 (70%) of MB samples. Neural stem cells had even lower levels of KCTD11 expression than did MB. HIC1 gene expression was low (∼100 times lower than KCTD11 expression) in MB, and low also in both adult cerebellum and neural stem cells. Hypermethylation of the 5′UTR or the central region of HIC1 (or both) was detected in a significant number of MB samples, as well as in cerebellum and neural stem cells. Our data suggest that KCTD11 may play an important role in MB tumorigenesis, but do not support the role of HIC1 in this tumor development. We argue that recognition of the gene or genes important in MB tumorigenesis depends in part on defining an appropriate control.
ISSN:0165-4608
1873-4456
DOI:10.1016/j.cancergencyto.2006.04.014