Overlapping expression patterns of the multiligand endocytic receptors cubilin and megalin in the CNS, sensory organs and developing epithelia of the rodent embryo

Cubilin and megalin are multiligand epithelial endocytic receptors well characterized in the adult kidney and ileum where they form a complex essential for protein, lipid and vitamin uptake. Although inactivation of the megalin gene leads to holoprosencephaly and administration of anti-cubilin antib...

Full description

Saved in:
Bibliographic Details
Published in:Gene Expression Patterns 2005-12, Vol.6 (1), p.69-78
Main Authors: Assémat, Emeline, Châtelet, François, Chandellier, Jacqueline, Commo, Frédéric, Cases, Olivier, Verroust, Pierre, Kozyraki, Renata
Format: Article
Language:English
Subjects:
Animals
Brain - embryology
Brain - metabolism
Central Nervous System - chemistry
Central Nervous System - embryology
Central Nervous System - metabolism
Cubilin
Ear - embryology
Embryo, Mammalian - chemistry
Embryo, Mammalian - metabolism
Endocytosis
Epithelia
Epithelium - embryology
Epithelium - metabolism
Eye - chemistry
Eye - embryology
Eye - metabolism
Female
Gastrointestinal Tract - chemistry
Gastrointestinal Tract - embryology
Gastrointestinal Tract - metabolism
Immunochemistry
In Situ Hybridization
Low Density Lipoprotein Receptor-Related Protein-2 - analysis
Low Density Lipoprotein Receptor-Related Protein-2 - genetics
Low Density Lipoprotein Receptor-Related Protein-2 - metabolism
Megalin
Multiligand endocytic receptors
Nose - chemistry
Nose - embryology
Nose - metabolism
Parathyroid Glands - chemistry
Parathyroid Glands - embryology
Parathyroid Glands - metabolism
Rats - embryology
Rats - genetics
Rats - metabolism
Rats, Wistar
Receptors, Cell Surface - analysis
Receptors, Cell Surface - genetics
Receptors, Cell Surface - metabolism
Respiratory System - chemistry
Respiratory System - embryology
Respiratory System - metabolism
Sense Organs - chemistry
Sense Organs - embryology
Sense Organs - metabolism
Sensory organs
Spinal Cord - chemistry
Spinal Cord - embryology
Spinal Cord - metabolism
Thymus Gland - chemistry
Thymus Gland - embryology
Thymus Gland - metabolism
Thyroid Gland - chemistry
Thyroid Gland - embryology
Thyroid Gland - metabolism
Tissue Distribution
Urogenital System - chemistry
Urogenital System - embryology
Urogenital System - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cubilin and megalin are multiligand epithelial endocytic receptors well characterized in the adult kidney and ileum where they form a complex essential for protein, lipid and vitamin uptake. Although inactivation of the megalin gene leads to holoprosencephaly and administration of anti-cubilin antibodies induces fetal resorptions or cranio-facial malformations their function in the developing embryo remains unclear. We recently showed that both proteins are strongly expressed by the maternal–fetal interfaces and the neuroepithelium of the early rodent embryo where they co-localize and form a complex important for nutrient uptake. The aim of the present study was the further investigation of cubilin expression at later developmental stages of the rodent embryo and its correlation to that of megalin. Immunohistochemical and in situ hybridization analysis showed striking similarities in the spatial and temporal expression patterns of cubilin and megalin. The electrophoretic mobility of both proteins was identical to that of the adult as revealed by Western blot analysis. Cubilin and megalin were strongly expressed in the sensory organs, the central nervous system, the respiratory and urogenital tracts as well as in the thymus, parathyroids and thyroid. In each site, the expression mainly concerned epithelial structures and correlated with the onset of epithelial induction. Depending on the site, a decreased or restricted expression was observed by the end of the gestation for both proteins.
ISSN:1567-133X
1872-7298
DOI:10.1016/j.modgep.2005.04.014