Effects of glucose limitation on biomass and spiramycin production by Streptomyces ambofaciens

Spiramycin production by Streptomyces ambofaciens Sp181110 with glucose as the carbon source was studied under a controlled nutritional environment. In a batch culture, the glucose excess after ammonium depletion led to pyruvate and α-ketoglutarate accumulation. 85 mg/l of spiramycin were produced i...

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Bibliographic Details
Published in:Bioprocess and biosystems engineering 2005-11, Vol.28 (1), p.55-61
Main Authors: Colombié, V, Bideaux, C, Goma, G, Uribelarrea, J. L
Format: Article
Language:English
Subjects:
alpha-ketoglutaric acid
Bacteria
Biological and medical sciences
Biomass
Bioreactors - microbiology
Biotechnology
carbon
Cell Culture Techniques - methods
Cell Proliferation
Computer Simulation
Fundamental and applied biological sciences. Psychology
glucose
Glucose - metabolism
Microbiology
Models, Biological
pyruvic acid
Species Specificity
spiramycin
Spiramycin - biosynthesis
Streptomyces - classification
Streptomyces - growth & development
Streptomyces - metabolism
Streptomyces ambofaciens
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Summary:Spiramycin production by Streptomyces ambofaciens Sp181110 with glucose as the carbon source was studied under a controlled nutritional environment. In a batch culture, the glucose excess after ammonium depletion led to pyruvate and α-ketoglutarate accumulation. 85 mg/l of spiramycin were produced in less than 70 h during the stationary and maintenance phase on these acids after glucose exhaustion. Fed-batch strategy was designed to study spiramycin production without by-product formation and glucose accumulation. In these conditions, up to 150 mg/l were produced in less than 80 h during the stationary phase on glucose. The antibiotic titre was found independent of the glucose feeding under carbon limitation and the importance of putative intracellular reserves formed after nutrient exhaustion was suggested. Besides, spiramycin production was not inhibited by the limiting flux of glucose.
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-005-0015-2