Using Bicellar Mixtures To Form Supported and Suspended Lipid Bilayers on Silicon Chips

Bicellar mixtures, planar lipid bilayer assemblies comprising long- and short-chain phosphatidylcholine lipids in suspension, were used to form supported lipid bilayers on flat silicon substrate and on nanotextured silicon substrates containing arrays of parallel troughs (170 nm wide, 380 nm deep, a...

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Bibliographic Details
Published in:Langmuir 2006-09, Vol.22 (19), p.8163-8168
Main Authors: Zeineldin, Reema, Last, Julie A, Slade, Andrea L, Ista, Linnea K, Bisong, Paul, O'Brien, Michael J, Brueck, S. R. J, Sasaki, Darryl Y, Lopez, Gabriel P
Format: Article
Language:English
Subjects:
Chemistry
Colloidal state and disperse state
Exact sciences and technology
General and physical chemistry
Lipid Bilayers
Membranes
Microscopy, Atomic Force
Microscopy, Confocal
Nanotechnology
Phosphatidylcholines - chemistry
Porosity
Porous materials
Silicon - chemistry
Surface Properties
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Summary:Bicellar mixtures, planar lipid bilayer assemblies comprising long- and short-chain phosphatidylcholine lipids in suspension, were used to form supported lipid bilayers on flat silicon substrate and on nanotextured silicon substrates containing arrays of parallel troughs (170 nm wide, 380 nm deep, and 300 nm apart). Confocal fluorescence and atomic force microscopies were used to characterize the resulting lipid bilayer. Formation of a continuous biphasic undulating lipid bilayer membrane, where the crests and troughs corresponded to supported and suspended lipid bilayer regions, is demonstrated. The use of interferometric lithography to fabricate nanotexured substrates provides an advantage over other nanotextured substrates such as nanoporous alumina by offering flexibility in designing different geometries for suspending lipid bilayers.
ISSN:0743-7463
1520-5827
DOI:10.1021/la060817r