Adipose tissue of human omentum is a major source of dendritic cells, which lose MHC Class II and stimulatory function in Crohn’s disease

Adipose tissue is reported to contain monocyte‐like pre‐adipocytes, which may mature into macrophages, contributing to local inflammation. Dendritic cells (DC) can be derived from monocytes and initiate and regulate primary immune responses. We hypothesized, therefore, that adipose tissue may provid...

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Bibliographic Details
Published in:Journal of leukocyte biology 2006-09, Vol.80 (3), p.546-554
Main Authors: Bedford, Penelope A., Todorovic, Vesna, Westcott, Edward D. A., Windsor, Alistair C. J., English, Nicholas R., Al‐Hassi, Hafid Omar, Raju, Kankipati S., Mills, Sarah, Knight, Stella C.
Format: Article
Language:English
Subjects:
Adipose Tissue - immunology
Adult
Aged
Biopsy - methods
Cell Movement - immunology
Cell Proliferation
Crohn Disease - diagnosis
Crohn Disease - immunology
Crohn Disease - pathology
Dendritic Cells - immunology
fatty acids
Histocompatibility Antigens Class II - immunology
Humans
inflammatory bowel disease
Middle Aged
mucosa
Omentum - immunology
Omentum - pathology
T-Lymphocytes, Cytotoxic - immunology
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Summary:Adipose tissue is reported to contain monocyte‐like pre‐adipocytes, which may mature into macrophages, contributing to local inflammation. Dendritic cells (DC) can be derived from monocytes and initiate and regulate primary immune responses. We hypothesized, therefore, that adipose tissue may provide DC involved in local immune activity. To test this, we studied cells from human omental adipose tissue samples from 17 patients with benign gynecological disease. The hypothesis that adipose tissue DC are involved in inflammatory disease was tested by comparing these cells with those from 18 patients with Crohn's disease, where hypertrophy of adipose tissue suggests involvement in disease. A high proportion of the 1.33 ± 0.12 × 105 CD45‐positive cells/mg, obtained from control omenta, expressed CD11c, CD1a, and CD83; costimulatory molecules CD40, CD80, and CD86; and major histocompatibility complex (MHC) Class II but little CD14, CD16, or CD33. Omental cells showing morphological characteristics of DC were also observed. Metrizamide gradient‐enriched DC from these populations were potent stimulators of primary proliferation of allogeneic T cells in mixed leukocyte reactions. Increased numbers of CD45+ cells from omentum of Crohn's patients (4.50±1.08×105 CD45+ cells/mg) contained higher percentages of CD11c+ and CD40+ cells (80.8±3.8% vs. 63.4±6, P=0.032; 77.9±4% vs. 58.8±6.5, P=0.029, respectively), but MHC Class II and stimulatory capacity were almost completely lost (P=
ISSN:0741-5400
1938-3673
DOI:10.1189/jlb.0905501