Characterization of blood components prepared from whole-blood donations after a 24-hour hold with the platelet-rich plasma method

BACKGROUND: The preparation of platelet (PLT) concentrates (PCs) from PLT‐rich plasma (PRP) requires that whole blood (WB) be processed within 8 hours of collection. Increasing WB storage time to 24 hours would be logistically attractive. This study compares the in vitro quality of blood components...

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Bibliographic Details
Published in:Transfusion (Philadelphia, Pa.) Pa.), 2006-08, Vol.46 (8), p.1292-1299
Main Authors: Thibault, Louis, Beauséjour, Annie, De Grandmont, Marie Joëlle, Lemieux, Réal, Leblanc, Jean-François
Format: Article
Language:English
Subjects:
ABO Blood-Group System
Adenosine Triphosphate - blood
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Biological and medical sciences
Blood Coagulation Factors - analysis
Blood coagulation. Blood cells
Blood Donors
Blood Glucose - analysis
Blood Platelets - metabolism
Blood Specimen Collection - methods
Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis
E-Selectin - blood
Emergency and intensive cardiocirculatory care. Cardiogenic shock. Coronary intensive care
Female
Fundamental and applied biological sciences. Psychology
Hemolysis
Humans
Intensive care medicine
Leukocyte Reduction Procedures - methods
Leukocytes - metabolism
Male
Medical sciences
Molecular and cellular biology
Plasma
Platelet
Time Factors
Transfusions. Complications. Transfusion reactions. Cell and gene therapy
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Summary:BACKGROUND: The preparation of platelet (PLT) concentrates (PCs) from PLT‐rich plasma (PRP) requires that whole blood (WB) be processed within 8 hours of collection. Increasing WB storage time to 24 hours would be logistically attractive. This study compares the in vitro quality of blood components prepared from WB stored for 8 and 24 hours at room temperature before processing with the PRP method. STUDY DESIGN AND METHODS: WB units were collected from ABO‐matched blood donors. To reduce individual variations, paired donations were drawn in parallel, pooled, and split back in the collection bag. One unit was held for 6 to 8 hours and the other for 22 to 24 hours at 20 to 24°C. Prestorage leukoreduced components were prepared with the PRP as intermediate product and analyzed during storage. RESULTS: RBC units prepared after an 8‐ or 24‐hour hold were comparable in terms of hemolysis, sodium, pH, and ATP levels. RBC 2,3‐ diphosphoglycerate (2,3‐DPG) was significantly lower in RBCs prepared from 24‐hour hold donations immediately after processing but not after 20 days of storage. Residual white blood cells were approximately fivefold higher (p 
ISSN:0041-1132
1537-2995
DOI:10.1111/j.1537-2995.2006.00894.x