Cecal parameters of rats fed diets containing grapefruit polyphenols and inulin as single supplements or in a combination

We compared the effects of grapefruit flavonoids and inulin, as single dietary components or in a combination, on cecal fermentation in rats adapted to a semipurified diet. The experimental diets contained 0.3% flavonoid extract and 5% or 10% inulin and a combination of both supplements. The large b...

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Published in:Nutrition (Burbank, Los Angeles County, Calif.) Los Angeles County, Calif.), 2006-09, Vol.22 (9), p.898-904
Main Authors: Zduńczyk, Zenon, Juśkiewicz, Jerzy, Estrella, Isabel
Format: Article
Language:English
Subjects:
Adaptation, Physiological
animal models
Animals
beta-Glucosidase - metabolism
Biological and medical sciences
Cecum
Cecum - chemistry
Cecum - metabolism
Cecum - microbiology
Citrus paradisi - chemistry
Diet
dietary fiber
Dietary Supplements
Digesta
Digestion - drug effects
Digestion - physiology
Dose-Response Relationship, Drug
Drug Combinations
Enzyme activity
experimental diets
Fatty Acids, Volatile - biosynthesis
Feeding. Feeding behavior
Fermentation - drug effects
Fermentation - physiology
flavonoids
Flavonoids - administration & dosage
Flavonoids - pharmacology
food intake
Fundamental and applied biological sciences. Psychology
Galactosidases - metabolism
Grapefruit polyphenols
grapefruits
Hydrogen-Ion Concentration
intestinal microorganisms
Inulin
Inulin - administration & dosage
Inulin - pharmacology
Male
Metabolites
Phenols - administration & dosage
Phenols - pharmacology
Polyphenols
Random Allocation
Rat
Rats
Rats, Wistar
Rodents
rumen fermentation
short chain fatty acids
Short-chain fatty acid
Vertebrates: anatomy and physiology, studies on body, several organs or systems
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Summary:We compared the effects of grapefruit flavonoids and inulin, as single dietary components or in a combination, on cecal fermentation in rats adapted to a semipurified diet. The experimental diets contained 0.3% flavonoid extract and 5% or 10% inulin and a combination of both supplements. The large bowel metabolism assessment was based on cecal parameters: bulk effect, pH, microbial enzymes activity, and short-chain fatty acid production. Both supplements induced significant enlargement of the cecal digesta weight. Acidification of cecal digesta was more pronounced, with a higher inulin addition to the diet. Cecal pH was the highest with the flavonoid-rich diets and lowest in the case of a simultaneous addition of flavonoids and a high content of inulin. The flavonoid extract applied as a single dietary supplement was observed to decrease the activity of bacterial β-glucosidase and β- and α-galactosidases in the cecal digesta. In contrast, addition of the grapefruit extract to inulin-containing diets increased the activity of α-glucosidase, α-galactosidase, and β-galactosidase. Great accumulation of cecal digesta in rats consuming the flavonoid-diet caused a considerable increase in the short-chain fatty acid pool, mainly acetic acid. Inulin added to the diet decreased the excessive enlargement of digesta caused by dietary flavonoids. Dietary addition of inulin to the flavonoid-diet also normalized hydration of cecal digesta and significantly decreased the pH of digesta. The presence of polyphenols in the inulin-containing diets did not change total short-chain fatty acid production in the cecum of rats. Our results suggested that simultaneous intake of inulin and polyphenols can decrease the detrimental effects of the latter on cecal fermentation.
ISSN:0899-9007
1873-1244
DOI:10.1016/j.nut.2006.05.010