Analysis of the Presence of ail, ystA and ystB Genes in Yersinia enterocolitica Strains Isolated from Aborting Sows and Aborted Fetuses

Forty-five Yersinia enterocolitica strains isolated from aborted fetuses and placentas and from vaginal and rectal swabs of aborting sows were subjected to serotyping, biochemical typing and polymerase chain reaction multiplex analyses to detect the presence of the ail, yst A and ystB genes. The iso...

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Bibliographic Details
Published in:Journal of veterinary medicine. Series B 2006-09, Vol.53 (7), p.341-346
Main Authors: Platt-Samoraj, A, Ugorski, M, Szweda, W, Szczerba-Turek, A, Wojciech, K, Procajło, Z
Format: Article
Language:English
Subjects:
Aborted Fetus - microbiology
abortion (animals)
Abortion, Veterinary - microbiology
ail gene
animal pathogenic bacteria
Animals
Bacteria
Bacterial Outer Membrane Proteins - genetics
Bacterial Outer Membrane Proteins - isolation & purification
Bacterial Toxins - genetics
Bacterial Toxins - isolation & purification
Biochemistry
biotypes
Cattle
Enterotoxins - genetics
Enterotoxins - isolation & purification
Enzymes
Female
fetal death
fetus
Fetuses
genes
Genetics
genotype
microbial genetics
placenta
Placenta - microbiology
Polymerase Chain Reaction - methods
Polymerase Chain Reaction - veterinary
Pregnancy
rectum
serotypes
Serotyping - veterinary
sows
strain differences
strains
Swine
swine diseases
vagina
Yersinia enterocolitica
Yersinia enterocolitica - genetics
Yersinia enterocolitica - isolation & purification
yersiniosis
ystA gene
ystB gene
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Summary:Forty-five Yersinia enterocolitica strains isolated from aborted fetuses and placentas and from vaginal and rectal swabs of aborting sows were subjected to serotyping, biochemical typing and polymerase chain reaction multiplex analyses to detect the presence of the ail, yst A and ystB genes. The isolates were recovered from the internal organs (tonsil, lung, liver, spleen, kidney, mesentheric lymph nodes, small intestine and rectal intestine) of 18 (18.6%) of 97 aborted fetuses examined, two (8%) of 25 aborted placentas and 27 (15.8%) of 172 examined aborting sows. Serotyping of Y. enterocolitica revealed that only six (13.3%) of the examined isolates belonged to serotype O:3, with a considerable number of isolates (31.1%) having serotype O:5, while biochemical studies showed that as many as 40 of the 45 strains belonged to biotype 1A. As expected, the Y. enterocolitica strains of bioserotype 4/O:3 contained ail and ystA genes, while strains of biotype 1A contained only the ystB gene.
ISSN:0931-1793
1863-1959
1439-0450
1863-2378
DOI:10.1111/j.1439-0450.2006.00969.x