Does Anti-Oxidant Prophylaxis with Melatonin Prevent Adverse Outcomes Related to Increased Oxidative Stress Caused by Laparoscopy in Experimental Rat Model?

We hypothesized that prophylaxis with an anti-oxidant should prevent potential adverse outcomes of laparoscopy related to increased oxidative stress in splanchnic organs, including small intestine, liver, and kidneys, and melatonin is the most appropriate agent for this purpose. Twenty-four Sprague-...

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Bibliographic Details
Published in:The Journal of surgical research 2006-09, Vol.135 (1), p.2-8
Main Authors: Çay, Ali, İmamoğlu, Mustafa, Ünsal, Mesut A., Aydin, Sevim, Alver, Ahmet, Akyol, Ahmet, Sarihan, Haluk
Format: Article
Language:English
Subjects:
Animals
Antioxidants - pharmacology
Biological and medical sciences
Digestive system. Abdomen
Disease Models, Animal
Endoscopy
General aspects
increased abdominal pressure
intestine
Intestine, Small - metabolism
Intestine, Small - pathology
Investigative techniques, diagnostic techniques (general aspects)
ischemia
Kidney - metabolism
Kidney - pathology
laparoscopy
Laparoscopy - adverse effects
Liver - metabolism
Liver - pathology
Male
Malondialdehyde - metabolism
Medical sciences
melatonin
Melatonin - pharmacology
Oxidative Stress - drug effects
Postoperative Complications - drug therapy
Postoperative Complications - pathology
Postoperative Complications - prevention & control
Pressure
Rats
Rats, Sprague-Dawley
reperfusion
Reperfusion Injury - drug therapy
Reperfusion Injury - pathology
Reperfusion Injury - prevention & control
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Summary:We hypothesized that prophylaxis with an anti-oxidant should prevent potential adverse outcomes of laparoscopy related to increased oxidative stress in splanchnic organs, including small intestine, liver, and kidneys, and melatonin is the most appropriate agent for this purpose. Twenty-four Sprague-Dawley rats weighing 300 to 350 g were allocated randomly into three groups consisting of eight in each as follows: Group I: gasless (control); group II: 15 mmHg intraabdominal pressure (IAP) with CO 2 pneumoperitoneum for 60 min; group III: 15 mmHg IAP with CO 2 pneumoperitoneum for 60 min, and melatonin (10 mg/kg) was administered at two occasions, 5 min before insufflation and immediately before the desufflation. In group II and III, rats left resting for 30 min after abdominal deflation, the small intestine (terminal ileum), liver and kidney examples were excised from same locations. The specimens were also obtained using the same time points in group I rats, comprising the control group. The specimens were immediately placed at −80°C for the malondialdehyde (MDA) measurements. In addition, segments of terminal ileum were taken from the similar places in all of the animals for the histological examinations. Comparisons among the groups revealed that highest mean MDA levels in liver, small intestine and kidney were in the group II, followed by the group III and control group. There was significant difference between mean MDA levels in small intestine, liver and kidney of group II and III ( P < 0.0005). However, no significant difference was found between mean MDA levels in small intestine, liver, and kidney of the group III and control group. Mucosa and submucosa were affected significantly in 15 mmHg IAP group (no prophylaxis) when compared with the control and melatonin prophylaxis groups ( P = 0.002). However, there was not a significant difference in mean damage score of mucosa, submucosa, and muscular layers in control group when compared to melatonin prophylaxis group. This experimental study indicated that melatonin prophylaxis, with anti-oxidant and anti-inflammatory actions, may have an important role in the prevention of potential complications related to oxidative stress injury on splanchnic organs induced by laparoscopy.
ISSN:0022-4804
1095-8673
DOI:10.1016/j.jss.2005.12.025