Sleep deprivation suppresses neurogenesis in the adult hippocampus of rats

We reported previously that 96 h of sleep deprivation (SD) reduced cell proliferation in the dentate gyrus (DG) of the hippocampus in adult rats. We now report that SD reduces the number of new cells expressing a mature neuronal marker, neuronal nuclear antigen (NeuN). Rats were sleep‐deprived for 9...

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Published in:The European journal of neuroscience 2005-10, Vol.22 (8), p.2111-2116
Main Authors: Guzman-Marin, Ruben, Suntsova, Natalia, Methippara, Melvi, Greiffenstein, Richard, Szymusiak, Ronald, McGinty, Dennis
Format: Article
Language:English
Subjects:
Animals
Behavior, Animal
BrdU
Bromodeoxyuridine - metabolism
Cell Count - methods
Cell Proliferation
dentate gyrus
hippocampus
Hippocampus - pathology
Immunohistochemistry - methods
Male
neurogenesis
Neurons - pathology
Phosphopyruvate Hydratase - metabolism
plasticity
Rats
Rats, Sprague-Dawley
sleep deprivation
Sleep Deprivation - physiopathology
Time Factors
Wakefulness - physiology
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Summary:We reported previously that 96 h of sleep deprivation (SD) reduced cell proliferation in the dentate gyrus (DG) of the hippocampus in adult rats. We now report that SD reduces the number of new cells expressing a mature neuronal marker, neuronal nuclear antigen (NeuN). Rats were sleep‐deprived for 96 h, using an intermittent treadmill system. Total sleep time was reduced to 6.9% by this method in SD animals, but total treadmill movement was equated in SD and treadmill control (CT) groups. Rats were allowed to survive for 3 weeks after 5‐bromo‐2‐deoxyuridine (BrdU) injection. The phenotype of BrdU‐positive cells in the DG was assessed by immunofluorescence and confocal microscopy. After 3 weeks the number of BrdU‐positive cells was reduced by 39.6% in the SD group compared with the CT. The percentage of cells that co‐localized BrdU and NeuN was also lower in the SD group (SD: 46.6 ± 1.8% vs. CT: 71.9 ± 2.1, P 
ISSN:0953-816X
1460-9568
DOI:10.1111/j.1460-9568.2005.04376.x