Establishment and characterization of renal progenitor like cells from S3 segment of nephron in rat adult kidney

Kidney is thought to be a regenerative organ in terms of repair from acute tubular injury. It is unknown whether cell population contributes to repair disordered kidney. We attempted to identify and isolate highly proliferative cells from a single cell. We dissected a single nephron from adult rat k...

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Bibliographic Details
Published in:The FASEB journal 2005-11, Vol.19 (13), p.1789-1797
Main Authors: Kitamura, Shinji, Yamasaki, Yasushi, Kinomura, Masaru, Sugaya, Takeshi, Sugiyama, Hitoshi, Maeshima, Yohei, Makino, Hirofumi
Format: Article
Language:English
Subjects:
Animals
Aquaporin 1 - biosynthesis
Aquaporin 2 - biosynthesis
Arginine Vasopressin - pharmacology
Cell Culture Techniques - methods
Cell Differentiation
Cell Line
Cell Proliferation
Cells, Cultured
Cyclic AMP - metabolism
Electrophoresis, Polyacrylamide Gel
Epithelial Cells - cytology
Immunoblotting
Karyotyping
Keratins - metabolism
Kidney - metabolism
Kidney - pathology
Kidney Diseases - pathology
Kidney Tubules - cytology
Kidney Tubules - metabolism
Kidney Tubules - pathology
Male
Microscopy, Fluorescence
Nephrons - cytology
Nephrons - pathology
Parathyroid Hormone - metabolism
Parathyroid Hormone - pharmacology
Phenotype
Rats
Rats, Sprague-Dawley
Regeneration
Renal Insufficiency - pathology
renal progenitor‐like cell
renal tubular epithelial cells
Reperfusion Injury
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
S3 segment of proximal tubules
Stem Cells - cytology
Time Factors
Vasopressins - chemistry
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Summary:Kidney is thought to be a regenerative organ in terms of repair from acute tubular injury. It is unknown whether cell population contributes to repair disordered kidney. We attempted to identify and isolate highly proliferative cells from a single cell. We dissected a single nephron from adult rat kidney. Isolated nephrons were separated into segments and cultured. Outgrowing cells were replated after limiting dilution so that each well contained a single cell. One of cell line which was the most potent to grow was designated as rKS56. rKS56 cells showed cobblestone appearance and expressed immature cell markers relating to kidney development and mature tubular cell markers. rKS56 cells grew exponentially and could be maintained for 300 days without transformation. In different culture conditions, rKS56 cells differentiated into mature tubular cells defined by aquaporin-1, 2 expression, and responsiveness to parathyroid hormone or vasopressin. Engrafted to kidney in rat ischemic reperfusion model, rKS56 cells replaced in injured tubules in part after implantation and improved renal function. These results suggest rKS56 cells possess character such as self-renewal, multi-plasticity and capability of tissue repair. rKS56 may possibly contribute to the future development of cell therapy for renal regeneration.--Kitamura, S., Yamasaki, Y., Kinomura, M., Sugaya, T., Sugiyama, H., Maeshima, Y., Makino, H. Establishment and characterization of renal progenitor like cells from S3 segment of nephron in rat adult kidney.
ISSN:0892-6638
1530-6860
DOI:10.1096/fj.05-3942com