Taurine transporter is expressed in osteoblasts

Taurine influences bone metabolism and is taken up by cells via a specific transport system, the taurine transporter (TAUT). We report a link between taurine and bone homeostasis by demonstrating transcription and translation of TAUT in bone-forming cells. TAUT was expressed in human primary osteobl...

Full description

Saved in:
Bibliographic Details
Published in:Amino acids 2006-08, Vol.31 (2), p.157-163
Main Authors: Yuan, L.-Q, Xie, H, Luo, X.-H, Wu, X.-P, Zhou, H.-D, Lu, Y, Liao, E.-Y
Format: Article
Language:English
Subjects:
3T3 Cells
alkaline phosphatase
Alkaline Phosphatase - metabolism
Animals
Base Sequence
Biocompatibility
Biomedical materials
Blotting, Western
Bones
Cell Differentiation
Culture
DNA Primers
Homeostasis
Human
Humans
Immunohistochemistry
Membrane Glycoproteins - genetics
Membrane Glycoproteins - metabolism
Membrane Transport Proteins - genetics
Membrane Transport Proteins - metabolism
Mice
Osteoblast
Osteoblasts
Osteoblasts - enzymology
Osteoblasts - metabolism
osteocalcin
Osteocalcin - secretion
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
taurine
Taurine transport
Transporter
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Taurine influences bone metabolism and is taken up by cells via a specific transport system, the taurine transporter (TAUT). We report a link between taurine and bone homeostasis by demonstrating transcription and translation of TAUT in bone-forming cells. TAUT was expressed in human primary osteoblasts, the human osteosarcoma osteoblast-like cell line MG63, and the mouse osteoblastic cell line MC3T3-E1. Immunostaining with polyclonal antibodies also demonstrated the presence of TAUT in both human and murine osteoblasts. TAUT mRNA expression and [³H]taurine uptake increased during differentiation of MG63 cells in culture. Supplementation of culture medium with taurine enhanced alkaline phosphatase activity and osteocalcin secretion. The regulation and detailed function of taurine and TAUT in bone remain unclear, but our findings suggest a functional role for them in bone homeostasis.
ISSN:0939-4451
1438-2199
DOI:10.1007/s00726-005-0313-7