Production of organic acids by Corynebacterium glutamicum under oxygen deprivation

Under oxygen deprivation, aerobic Corynebacterium glutamicum produce organic acids from glucose at high yields in mineral medium even though their proliferation is arrested. To develop a new, high-productivity bioprocess based on these unique features, characteristics of organic acid production by C...

Full description

Saved in:
Bibliographic Details
Published in:Applied microbiology and biotechnology 2005-09, Vol.68 (4), p.475-480
Main Authors: Okino, Shohei, Inui, Masayuki, Yukawa, Hideaki
Format: Article
Language:English
Subjects:
Acids
Bacteria
Biological and medical sciences
Biotechnology
Corynebacterium glutamicum
Corynebacterium glutamicum - metabolism
Fundamental and applied biological sciences. Psychology
Glucose
Hypoxia - metabolism
Lactic Acid - metabolism
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Microbiology
Succinic Acid - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Under oxygen deprivation, aerobic Corynebacterium glutamicum produce organic acids from glucose at high yields in mineral medium even though their proliferation is arrested. To develop a new, high-productivity bioprocess based on these unique features, characteristics of organic acid production by C. glutamicum under oxygen deprivation were investigated. The main organic acids produced from glucose under these conditions were lactic acid and succinic acid. Addition of bicarbonate, which is a co-substrate for anaplerotic enzymes, increased the glucose consumption rate, leading to increased organic acid production rates. With increasing concentration of bicarbonate, the yield of succinic acid increased, whereas that of lactic acid decreased. There was a direct correlation between cell concentration and organic acid production rates even at elevated cell densities, and productivities of lactic acid and succinic acid were 42.9 g l-¹ h-¹ and 11.7 g l-¹ h-¹, respectively, at a cell concentration of 60 g dry cell l-¹. This cell-recycling continuous reaction demonstrated that rates of organic acid production by C. glutamicum could be maintained for at least 360 h.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-005-1900-y