The N-acetyl- d-glucosamine specific adhesin from Mannheimia haemolytica activates bovine neutrophils oxidative burst

In this work we identified specific bovine leukocytes that were bound by the Mannheimia haemolytica adhesin molecule (MhA) and the biological effect on the leukocytes. Histochemical staining and flow cytometry showed that MhA bind neutrophils (90%) and monocytes (5%). MhA induced an oxidative respon...

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Published in:Veterinary Immunology and Immunopathology 2006-09, Vol.113 (1), p.148-156
Main Authors: De la Mora, Alfonso, Trigo, Francisco, Jaramillo, Laura, Garfias, Yonathan, Solórzano, Carlos, Agundis, Concepción, Pereyra, Ali, Lascurain, Ricardo, Zenteno, Edgar, Suárez-Güemes, Francisco
Format: Article
Language:English
Subjects:
Acetylglucosamine - immunology
Adhesin
adhesins
Adhesins, Bacterial - immunology
Adhesins, Bacterial - isolation & purification
Adhesins, Bacterial - pharmacology
animal pathogenic bacteria
Animals
Bovine
cattle
Cattle - blood
cattle diseases
Cattle Diseases - blood
Cattle Diseases - immunology
Cattle Diseases - microbiology
flow cytometry
Flow Cytometry - veterinary
free radicals
GlcNAc-specificity
histochemistry
Immunohistochemistry - veterinary
infection
Iodoacetamide - pharmacology
leukocytes
Mannheimia haemolytica
Mannheimia haemolytica - immunology
Mannheimia haemolytica - isolation & purification
Mannheimiosis
monocytes
N-acetylglucosamine
Neutrophil Activation - immunology
Neutrophils - drug effects
Neutrophils - immunology
Neutrophils - microbiology
Neutrophils oxidative burst
oxidation
Pasteurellosis, Pneumonic - immunology
Pasteurellosis, Pneumonic - microbiology
Piroxicam - pharmacology
pneumonic pasteurellosis
Respiratory Burst - drug effects
Respiratory Burst - immunology
Superoxide Dismutase - pharmacology
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Summary:In this work we identified specific bovine leukocytes that were bound by the Mannheimia haemolytica adhesin molecule (MhA) and the biological effect on the leukocytes. Histochemical staining and flow cytometry showed that MhA bind neutrophils (90%) and monocytes (5%). MhA induced an oxidative response in purified neutrophils; this effect was 1.5-fold higher than the effect observed with control cells activated with Zymosan. Cellular binding by MhA was inhibited with GlcNAc and its oligomers, as well as by glycoproteins containing tri- and tetra-antennary N-glycosydically linked glycans. MhA-induced oxidative burst was significantly inhibited by GlcNAc, iodoacetamide, superoxide dismutase, and piroxicam ( p < 0.05). Our findings suggest that among bovine leukocytes, neutrophils are the main target for MhA, inducing production of oxidative radicals by non-opsonic mechanism that seem to play an important role in tissue damage during mannheimiosis.
ISSN:0165-2427
1873-2534
1365-2567
DOI:10.1016/j.vetimm.2006.04.011