Class 1 integrons in ciprofloxacin-resistant Escherichia coli strains from two Dutch hospitals

A significant increase in the isolation frequency of ciprofloxacin-resistant Escherichia coli was observed in the haematology departments of two university hospitals in The Netherlands. Amplified fragment length polymorphism analysis revealed that this increase was not caused by the emergence of uni...

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Bibliographic Details
Published in:Clinical microbiology and infection 2005-11, Vol.11 (11), p.898-902
Main Authors: Mooij, M.J., Schouten, I., Vos, G., Van Belkum, A., Vandenbroucke-Grauls, C.M.J.E., Savelkoul, P.H.M., Schultsz, C.
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
Biological and medical sciences
Ciprofloxacin
Ciprofloxacin - pharmacology
Conjugation, Genetic
DNA Fingerprinting
DNA Gyrase - genetics
DNA, Bacterial - analysis
DNA, Bacterial - genetics
Drug Resistance, Bacterial
Escherichia coli
Escherichia coli - drug effects
Escherichia coli - genetics
Escherichia coli - isolation & purification
Escherichia coli Infections - microbiology
Escherichia coli Proteins - genetics
Gene Transfer, Horizontal
Hematologic Diseases - complications
Hospitals
Humans
Inpatients
Integrases - genetics
Integrons
Medical sciences
Netherlands
Nucleic Acid Hybridization
Pharmacology. Drug treatments
Polymorphism, Genetic
quinolone resistance
resistance
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Summary:A significant increase in the isolation frequency of ciprofloxacin-resistant Escherichia coli was observed in the haematology departments of two university hospitals in The Netherlands. Amplified fragment length polymorphism analysis revealed that this increase was not caused by the emergence of unique ciprofloxacin-resistant clones. Determination of the presence of class 1 integrons indicated that 81% of the ciprofloxacin-resistant isolates contained an intI1 gene, compared with 11% of the ciprofloxacin-susceptible isolates (p < 0.0001). The quinolone resistance gene qnrA was not present in any of the integrons characterised and could not be detected using dot-blot hybridisation of total DNA. In addition, conjugation experiments showed that ciprofloxacin resistance was not co-transferred with class 1 integrons. Ciprofloxacin-resistant isolates harboured mutations in the gyrA gene, which are known to encode ciprofloxacin resistance. In conclusion, an association was observed between ciprofloxacin resistance and the presence of class 1 integrons, which could not be explained by the currently known genetic determinants of quinolone resistance.
ISSN:1198-743X
1469-0691
DOI:10.1111/j.1469-0691.2005.01259.x