DNA microarray analysis of gene expression in eutopic endometrium from patients with deep endometriosis using laser capture microdissection

To investigate differentially expressed genes in epithelial and stromal cells of eutopic endometrium from patients with deep endometriosis and women with normal pelvic cavities using laser capture microdissection and complementary DNA microarrays. Prospective study. University hospital. Patients wit...

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Bibliographic Details
Published in:Fertility and sterility 2005-10, Vol.84, p.1180-1190
Main Authors: Matsuzaki, Sachiko, Canis, Michel, Vaurs-Barrière, Catherine, Boespflug-Tanguy, Odile, Dastugue, Bernard, Mage, Gérard
Format: Article
Language:English
Subjects:
cDNA microarray
endometriosis
Endometriosis - genetics
Endometriosis - metabolism
Endometriosis - pathology
endometrium
Endometrium - metabolism
Endometrium - pathology
Female
Gene Expression Profiling - methods
Humans
laser capture microdissection
Lasers
Microdissection - methods
Oligonucleotide Array Sequence Analysis - methods
Prospective Studies
Statistics, Nonparametric
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Summary:To investigate differentially expressed genes in epithelial and stromal cells of eutopic endometrium from patients with deep endometriosis and women with normal pelvic cavities using laser capture microdissection and complementary DNA microarrays. Prospective study. University hospital. Patients with deep endometriosis and fertile women who underwent laparoscopic tubal ligation or reversal of tubal sterilization. Endometrial tissue biopsies during the late proliferative phase and early, mid-, and late secretory phases. Genes that were regulated with a change greater than threefold were selected as differentially expressed genes. Validation was performed with real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Microarray analysis identified up-regulation during the late secretory phase (patients with endometriosis vs. controls) of several genes in two important signaling pathways: RAS/RAF/MAPK and PI3K. This included the genes RON, SOS, 14-3-3 protein eta, and uPAR in epithelial cells and KSR and PI3K p85 regulatory subunit alpha in stromal cells; real-time RT-PCR analysis validated up-regulation of all six genes. The RAS/RAF/MAPK and PI3K pathways may be involved in initial development of endometriosis.
ISSN:0015-0282
1556-5653
DOI:10.1016/j.fertnstert.2005.04.041