Poly(ADP-ribose)polymerase activity is reduced in circulating mononuclear cells from type 2 diabetic patients

Poly(ADP‐ribose)polymerase (PARP‐1), a nuclear enzyme activated by DNA strand breaks, is involved in DNA repair, aging, inflammation, and neoplastic transformation. In diabetes, reactive oxygen and nitrogen species occurring in response to hyperglycemia cause DNA damages and PARP‐1 activation. Becau...

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Published in:Journal of cellular physiology 2005-12, Vol.205 (3), p.387-392
Main Authors: Tempera, Italo, Cipriani, Rosalba, Campagna, Gianluca, Mancini, Patrizia, Gatti, Alessandra, Guidobaldi, Leo, Pantellini, Federico, Mandosi, Elisabetta, Sensi, Maurizio, Quesada, Piera, Mario, Umberto Di, D'Erme, Maria, Morano, Susanna
Format: Article
Language:English
Subjects:
Aged
Blotting, Western
Case-Control Studies
Caspase 3
Caspases - metabolism
Diabetes Mellitus, Type 2 - blood
Enzyme Activation
Female
Humans
Male
Microscopy, Fluorescence
Middle Aged
Monocytes - enzymology
Poly (ADP-Ribose) Polymerase-1
Poly(ADP-ribose) Polymerases - blood
Poly(ADP-ribose) Polymerases - genetics
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - blood
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Summary:Poly(ADP‐ribose)polymerase (PARP‐1), a nuclear enzyme activated by DNA strand breaks, is involved in DNA repair, aging, inflammation, and neoplastic transformation. In diabetes, reactive oxygen and nitrogen species occurring in response to hyperglycemia cause DNA damages and PARP‐1 activation. Because circulating mononuclear cells (MNCs) are involved in inflammation mechanisms, these cells were chosen as the experimental model to evaluate PARP‐1 levels and activity in patients with type 2 diabetes. MNCs were isolated from 25 diabetic patients (18 M, 7 F, age, 63.5 ± 10.2 years, disease duration 17.7 ± 8.2 years) and 11 age and sex matched healthy controls. PARP‐1 expression and activity were analyzed by semi‐quantitative PCR, Western and activity blot, and immunofluorescence microscopy. PARP‐1‐mRNA expression was increased in MNCs from all diabetic patients versus controls (P 
ISSN:0021-9541
1097-4652
DOI:10.1002/jcp.20414