Expression of p63 and Cytokeratin 5 in Mixed Tumors of the Canine Mammary Gland Provides New Insights into the Histogenesis of These Neoplasms

Cytokeratin 5 and p63 have been described as basal and myoepithelial cell markers in human breast. Mixed tumors of the canine mammary gland have been associated with a myoepithelial origin. Cytokeratin 5 expression has not been evaluated in these tumors. We investigated the relation between cytokera...

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Bibliographic Details
Published in:Veterinary pathology 2006-07, Vol.43 (4), p.424-429
Main Authors: Ramalho, L.N.Z, Ribeiro-Silva, A, Cassali, G.D, Zucoloto, S
Format: Article
Language:English
Subjects:
Animals
biomarkers
Biomarkers, Tumor - biosynthesis
Biomarkers, Tumor - genetics
Carcinoma - genetics
Carcinoma - metabolism
Carcinoma - pathology
Carcinoma - veterinary
Dog Diseases - genetics
Dog Diseases - metabolism
Dog Diseases - pathology
Dogs
Female
gene expression
Genes, Tumor Suppressor
immunohistochemistry
Immunohistochemistry - veterinary
Keratins - biosynthesis
Keratins - genetics
mammary neoplasms (animal)
Mammary Neoplasms, Animal - genetics
Mammary Neoplasms, Animal - metabolism
Mammary Neoplasms, Animal - pathology
Retrospective Studies
Tumor Suppressor Proteins - biosynthesis
Tumor Suppressor Proteins - genetics
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Summary:Cytokeratin 5 and p63 have been described as basal and myoepithelial cell markers in human breast. Mixed tumors of the canine mammary gland have been associated with a myoepithelial origin. Cytokeratin 5 expression has not been evaluated in these tumors. We investigated the relation between cytokeratin 5 and p63 double-immunohistochemical expression in 23 mixed tumors of the canine mammary gland (10 benign mixed tumors and 13 carcinomas arising from benign mixed tumors) and their origin. Cytokeratin 5 and p63 co-expression was observed in myoepithelial cells of benign mixed tumors, as well as in squamous differentiation of carcinoma arising from benign mixed tumors. Though a few interstitial spindle cells of the mesenchymal components expressed both p63 and cytokeratin 5, the basal epithelial cells were labeled only by cytokeratin 5. The co-expression of p63 and cytokeratin 5 in myoepithelial cells and squamous differentiation suggest that, like in human breast, cytokeratin 5 can also be considered a myoepithelial- and squamous-cell differentiating marker in canine tumors. The presence of some interstitial spindle cells stained for p63 and cytokeratin 5 might be associated with a myoepithelial origin of the mesenchymal component of mixed tumors of the canine mammary gland. Moreover, contrary to p63, basal epithelial cells were labeled by cytokeratin 5, indicating that cytokeratin 5 may not represent an exclusive myoepithelial cell marker but also a basal epithelial cell marker in canine mixed tumors. According to these data, basal epithelial cells may be related to the origin of the epithelial component of mixed tumors of the canine mammary gland.
ISSN:0300-9858
1544-2217
DOI:10.1354/vp.43-4-424