Electrochemical Interrogation of DNA Monolayers on Gold Surfaces

In this report, we systematically investigated DNA immobilization at gold surfaces with electrochemical techniques. Comparative cyclic voltammetric and chronocoulometric studies suggested that DNA monolayers immobilized at gold surfaces were not homogeneous. Nonspecific Au−DNA interactions existed e...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2005-10, Vol.77 (19), p.6475-6480
Main Authors: Lao, Ruojun, Song, Shiping, Wu, Haiping, Wang, Lihua, Zhang, Zhizhou, He, Lin, Fan, Chunhai
Format: Article
Language:English
Subjects:
Analytical chemistry
Biosensors
Chemistry
Deoxyribonucleic acid
DNA
DNA - chemistry
Electrochemical methods
Electrochemistry
Exact sciences and technology
General, instrumentation
Gold
Gold - chemistry
Hybridization
Nanoparticles
Surface Properties
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Summary:In this report, we systematically investigated DNA immobilization at gold surfaces with electrochemical techniques. Comparative cyclic voltammetric and chronocoulometric studies suggested that DNA monolayers immobilized at gold surfaces were not homogeneous. Nonspecific Au−DNA interactions existed even with the treatment of mercaptohexanol, which was known to competitively remove loosely bound DNA at gold surfaces. While both thiolated and nonthiolated DNA formed monolayers on gold surfaces, their hybridization abilities were distinctly different. In contrast to thiolated DNA probes, nonthiolated DNA probes immobilized at gold surfaces were essentially nonhybridizable. The experimental results presented here might be useful for the design of high-performance electrochemical DNA sensors.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac050911x