Rev1 Employs a Novel Mechanism of DNA Synthesis Using a Protein Template

The Rev1 DNA polymerase is highly specialized for the incorporation of C opposite template G. We present here the crystal structure of yeast Rev1 bound to template G and incoming 2'-deoxycytidine 5'-triphosphate (dCTP), which reveals that the polymerase itself dictates the identity of the...

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Bibliographic Details

Published in:	Science (American Association for the Advancement of Science) 2005-09, Vol.309 (5744), p.2219-2222
Main Authors:	Nair, Deepak T, Johnson, Robert E, Prakash, Louise, Prakash, Satya, Aggarwal, Aneel K
Format:	Article
Language:	eng
Subjects:	Active sites Adducts Analysis Arginine - metabolism Base Pairing Binding Sites Biological and medical sciences Catalytic Domain Crystal structure Crystalline structure Crystallography, X-Ray Deoxycytosine Nucleotides - chemistry Deoxycytosine Nucleotides - metabolism DNA DNA polymerase DNA Replication DNA synthesis DNA, Fungal - biosynthesis DNA-directed DNA polymerase enzyme activity Fingers Fundamental and applied biological sciences. Psychology Guanine - chemistry Guanine - metabolism Hydrogen Hydrogen Bonding Hydrogen bonds Hydrophobic and Hydrophilic Interactions Models, Molecular Molecular biophysics Molecules Nucleotides Nucleotidyltransferases - chemistry Nucleotidyltransferases - genetics Nucleotidyltransferases - metabolism Polls Protein Conformation Protein Structure, Secondary Protein Structure, Tertiary Proteins Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - chemistry Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Structure in molecular biology Templates, Genetic Thumb Yeast yeasts
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