Dissemination of SHV-12 and CTX-M-type extended-spectrum β-lactamases among clinical isolates of Escherichia coli and Klebsiella pneumoniae and emergence of GES-3 in Korea

Objectives: To assess the prevalence and genotypes of Ambler class A extended-spectrum β-lactamases (ESBLs) in Korea. Methods: Clinical isolates of Escherichia coli and Klebsiella pneumoniae collected from 12 Korean hospitals during February–July 2003 were evaluated. Antimicrobial susceptibilities w...

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Bibliographic Details
Published in:Journal of antimicrobial chemotherapy 2005-10, Vol.56 (4), p.698-702
Main Authors: Ryoo, Nam Hee, Kim, Eui-Chong, Hong, Seong Geun, Park, Yeon Joon, Lee, Kyungwon, Bae, Il Kwon, Song, Eun Hyang, Jeong, Seok Hoon
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Antibiotics. Antiinfectious agents. Antiparasitic agents
beta-Lactamases - genetics
Biological and medical sciences
Drug Resistance, Bacterial
ESBLs
Escherichia coli
Escherichia coli - drug effects
Escherichia coli - enzymology
Escherichia coli - genetics
Escherichia coli - isolation & purification
Escherichia coli Infections - epidemiology
Escherichia coli Infections - microbiology
Hospitals
Humans
Klebsiella Infections - epidemiology
Klebsiella Infections - microbiology
Klebsiella pneumoniae
Klebsiella pneumoniae - drug effects
Klebsiella pneumoniae - enzymology
Klebsiella pneumoniae - genetics
Klebsiella pneumoniae - isolation & purification
Korea - epidemiology
Medical sciences
Microbial Sensitivity Tests
Pharmacology. Drug treatments
prevalence
resistance
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Summary:Objectives: To assess the prevalence and genotypes of Ambler class A extended-spectrum β-lactamases (ESBLs) in Korea. Methods: Clinical isolates of Escherichia coli and Klebsiella pneumoniae collected from 12 Korean hospitals during February–July 2003 were evaluated. Antimicrobial susceptibilities were determined by disc diffusion and agar dilution methods, and the putative ESBL-producing strains were tested by the double-disc synergy method. Detection of genes encoding class A β-lactamases was performed by PCR amplification, and the PCR products were subjected to direct sequencing. Results: The double-disc synergy test showed positive results in 9.3% (23/246) of E. coli and 23.0% (55/239) of K. pneumoniae isolates. The most prevalent types of Ambler class A ESBLs in E. coli isolates were CTX-M-15 (n = 4) and CTX-M-3 (n = 3), and those in K. pneumoniae isolates were SHV-12 (n = 30) and CTX-M-3 (n = 13). Two isolates produced both SHV-12 and GES-3, simultaneously. Conclusions: CTX-M-type and/or SHV-12 ESBL-producing E. coli and K. pneumoniae isolates are spreading, and a GES-type ESBL has emerged in Korea.
ISSN:0305-7453
1460-2091
DOI:10.1093/jac/dki324