Differential expression of human leukocyte antigen-G (HLA-G) messenger RNAs and proteins in normal human prostate and prostatic adenocarcinoma

Human leukocyte antigen-G ( HLA- G) is a major histocompatibility complex class Ib gene expressed in normal organs and in some tumors. The glycoproteins encoded by this gene are best known for their immunosuppressive properties. Because isoform-specific expression of HLA-G in male reproductive organ...

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Bibliographic Details
Published in:Journal of Reproductive Immunology 2006-08, Vol.71 (1), p.75-86
Main Authors: Langat, Daudi K., Sue Platt, J., Tawfik, Ossama, Fazleabas, Asgerally T., Hunt, Joan S.
Format: Article
Language:English
Subjects:
Adenocarcinoma
Adenocarcinoma - genetics
Adenocarcinoma - metabolism
Adenocarcinoma - pathology
Biological and medical sciences
Embryology: invertebrates and vertebrates. Teratology
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Neoplastic - genetics
Health
Histocompatibility Antigens Class I - genetics
Histocompatibility Antigens Class I - metabolism
HLA Antigens - genetics
HLA Antigens - metabolism
HLA-G
HLA-G Antigens
Humans
Immunohistochemistry
Leukocytes - metabolism
Male
Pregnancy
Prostate
Prostate - metabolism
Prostate - pathology
Prostatic Neoplasms - genetics
Prostatic Neoplasms - metabolism
Prostatic Neoplasms - pathology
Reproduction
RNA, Messenger - genetics
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Summary:Human leukocyte antigen-G ( HLA- G) is a major histocompatibility complex class Ib gene expressed in normal organs and in some tumors. The glycoproteins encoded by this gene are best known for their immunosuppressive properties. Because isoform-specific expression of HLA-G in male reproductive organs has not been reported, we investigated HLA-G1, -G2, -G5, -G6 mRNAs and proteins in four-to-five samples of normal prostate glands, prostates with benign prostatic hyperplasia and prostate adenocarcinomas using RT-PCR and immunohistochemistry. All tissues contained HLA-G1, -G2, -G5 and -G6 specific mRNAs, but only HLA-G5 protein was detectable. In normal prostate glands, HLA-G5 protein was prominent in the cytoplasm of tubuloglandular epithelia and in glandular secretions. Staining was reduced in samples of benign prostatic hyperplasia but remained localized to the cytoplasm of glandular epithelia and secretions. In prostatic adenocarcinomas, HLA-G5 protein was detectable mainly in the secretions. Thus, HLA-G5 but not HLA-G1, -G2 or -G6 is produced in the normal prostate and is present in prostatic secretions. In addition, normal cellular localization is disturbed in benign and malignant prostatic adenocarcinomas. The results are consistent with this molecule may influencing female immune receptivity to sperm and suggest that such immunosuppression could be disturbed in men with prostatic adenocarcinomas.
ISSN:0165-0378
1872-7603
1365-2567
DOI:10.1016/j.jri.2006.01.006