Experimental model of primary cultured metastatic melanoma by fine needle aspiration

Establish an experimental model of metastatic melanoma primary culture cells from fine-needle aspiration biopsies (FNAB). Two metastatic melanoma primary culture cells from (FNAB) have been developed from patients who had been submitted to excision of metastatic lesions and they were identified by i...

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Bibliographic Details
Published in:Acta cirúrgica brasileira 2005-09, Vol.20 (5), p.390-393
Main Authors: Oliveira, Andrea Fernandes de, Gragnani, Alfredo, Oliveira Filho, Renato Santos, Santos, Ivan Dunshee de Abranches Oliveira, França, Silvana Gaiba de, Enokihara, Mílvia Maria Simões e Silva, Ferreira, Lydia Masako
Format: Article
Language:Portuguese
Subjects:
Animals
Biomarkers, Tumor - analysis
Biopsy, Needle - methods
Cattle
Cell Culture Techniques - methods
Humans
Immunohistochemistry
Lymph Node Excision
Lymph Nodes - pathology
Lymphatic Metastasis
Melanoma - pathology
Melanoma - secondary
S100 Proteins - analysis
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Summary:Establish an experimental model of metastatic melanoma primary culture cells from fine-needle aspiration biopsies (FNAB). Two metastatic melanoma primary culture cells from (FNAB) have been developed from patients who had been submitted to excision of metastatic lesions and they were identified by immunohistochemical analyses using S-100 and MB-45 melanoma markers. The culture diagnostic was confirmed by immunohistochemical. The technique of FNAB offers the advantage of providing a sequential analysis of the same tumor nodules throughout treatment, and is a minimally invasive procedure with almost no associated morbidity. The establishment of metastatic melanoma primary culture from FNAB samples showed a viable technique.
ISSN:0102-8650