A recombinant chimera composed of R1 repeat region of Mycoplasma hyopneumoniae P97 adhesin with Escherichia coli heat-labile enterotoxin B subunit elicits immune response in mice

Swine mycoplasmal pneumonia (SMP), caused by fastidious bacterium Mycoplasma hyopneumoniae, is the most important respiratory disease in swine breeding. The commonly used vaccines to control this disease consist of inactivated whole cells (bacterins), whose production cost is high and the efficiency...

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Bibliographic Details
Published in:Vaccine 2006-07, Vol.24 (29), p.5734-5743
Main Authors: Conceição, Fabricio Rochedo, Moreira, Ângela Nunes, Dellagostin, Odir Antônio
Format: Article
Language:English
Subjects:
Adhesins, Bacterial - chemistry
Adhesins, Bacterial - genetics
Adhesins, Bacterial - immunology
Animals
Antibodies, Bacterial - blood
Applied microbiology
Bacterial Toxins - genetics
Bacterial Toxins - immunology
Bacterial Toxins - metabolism
Bacterial Vaccines - administration & dosage
Bacterial Vaccines - immunology
Bacteriology
Biological and medical sciences
Enterotoxins - genetics
Enterotoxins - immunology
Enterotoxins - metabolism
Escherichia coli
Escherichia coli - immunology
Escherichia coli - metabolism
Escherichia coli Proteins - genetics
Escherichia coli Proteins - immunology
Escherichia coli Proteins - metabolism
Female
Fundamental and applied biological sciences. Psychology
Immunoglobulin A - blood
Immunoglobulin A - metabolism
Interferon-gamma - biosynthesis
LTB
Mice
Mice, Inbred BALB C
Microbiology
Miscellaneous
Mycoplasma hyopneumoniae
Mycoplasma hyopneumoniae - immunology
Mycoplasma hyopneumoniae - metabolism
P97
Pneumonia of Swine, Mycoplasmal - immunology
Pneumonia of Swine, Mycoplasmal - prevention & control
Recombinant chimera
Recombinant Fusion Proteins - immunology
Recombinant Fusion Proteins - metabolism
Repetitive Sequences, Nucleic Acid
Swine mycoplasmal pneumonia
T-Lymphocytes - immunology
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
Vaccines, Synthetic - administration & dosage
Vaccines, Synthetic - immunology
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Summary:Swine mycoplasmal pneumonia (SMP), caused by fastidious bacterium Mycoplasma hyopneumoniae, is the most important respiratory disease in swine breeding. The commonly used vaccines to control this disease consist of inactivated whole cells (bacterins), whose production cost is high and the efficiency is limited. The objective of this study was to develop and to evaluate in BALB/c mice a recombinant subunit vaccine (rLTBR1) containing the R1 region of P97 adhesin of M. hyopneumoniae (R1) fused to the B subunit of the heat-labile enterotoxin of Escherichia coli (LTB). rLTBR1 formed functional oligomers that presented high affinity to GM1 ganglioside. Mice inoculated with rLTBR1 by intranasal (IN) or intramuscular (IM) route produced high levels of anti-R1 systemic and mucosal antibodies (IgA), which recognized the native P97. On the other hand, mice inoculated with the inactivated whole cell vaccine did not produce anti-R1 antibodies. The administration route influenced the modulation of the immune response by LTB, showing that IM rLTBR1 induced Th2-biased immune responses and IN rLTBR1 induced Th1-biased immune responses. rLTBR1 administrated by IN route also induced IFN-γ secretion by lymphocytes. rLTBR1 may constitute a new strategy for preventing infection by M. hyopneumoniae and may have potential for developing vaccines against other infectious diseases as well.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2006.04.036