DNA fingerprinting pattern and susceptibility to antifungal drugs in Cryptococcus neoformans variety grubii isolates from Barcelona city and rural environmental samples

Cryptococcus neoformans var. grubii (serotype A) was isolated from 12 soil samples mixed with pigeon droppings (16.9%) from 71 soil samples in Barcelona and rural areas of Catalonia. C. neoformans was not isolated from indoor dust and Eucalyptus debris. PCR fingerprinting was performed in 22 represe...

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Bibliographic Details
Published in:Mycopathologia (1975) 2005-08, Vol.160 (1), p.9-14
Main Authors: Morera-López, Yolanda, Torres-Rodríguez, Josep Maria, Jiménez-Cabello, Teresa, Baró-Tomás, Teresa, Alía-Aponte, Concepción, Lázera, Marcia S
Format: Article
Language:English
Subjects:
Amphotericin B - pharmacology
Antifungal Agents - pharmacology
Cryptococcus neoformans
Cryptococcus neoformans - drug effects
Cryptococcus neoformans - genetics
Cryptococcus neoformans - isolation & purification
DNA Fingerprinting
DNA, Fungal - genetics
Drug Resistance, Fungal
Eucalyptus
Fluconazole - pharmacology
Itraconazole - pharmacology
Ketoconazole - pharmacology
Microbial Sensitivity Tests
Pyrimidines - pharmacology
Soil Microbiology
Spain
Triazoles - pharmacology
Voriconazole
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Summary:Cryptococcus neoformans var. grubii (serotype A) was isolated from 12 soil samples mixed with pigeon droppings (16.9%) from 71 soil samples in Barcelona and rural areas of Catalonia. C. neoformans was not isolated from indoor dust and Eucalyptus debris. PCR fingerprinting was performed in 22 representative isolates and all of them corresponded to the VNI pattern. Susceptibility testing for the 22 isolates of C. neoformans var. grubii showed that all of them were susceptible to amphotericin B. Three isolates presented MICs (Minimal Inhibitory Concentrations) > or = 1 microg/ml to Itraconazole, five MICs > or = 1 microg/ml to ketoconazole and four were fluconazole resistant, (MICs > or = 64 microg/ml), while three of them were shown to have MICs > or = 1 microg/ml to voriconazole. In spite that all isolates presented the same DNA fingerprinting pattern, the susceptibility to antifungals is very variable. The possibility of acquiring cryptococcosis infection with primarily resistant environment strains is feasible.
ISSN:0301-486X
1573-0832
DOI:10.1007/s11046-005-6332-9