A study on the photodynamic properties of chlorophyll derivatives using human hepatocellular carcinoma cells

Photodynamic therapy (PDT) is an alternative anticancer treatment in which direct tumor-cell killing results from selective accumulation of photosensitizers in the tumor sites and phototoxicity occurs when light-activated photosensitizers transfer the energy to oxygen nearby to produce singlet oxyge...

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Bibliographic Details
Published in:Photochemical & photobiological sciences 2007-12, Vol.6 (12), p.1341-1348
Main Authors: Li, Wen-Tyng, Tsao, Hsin-Wei, Chen, Ying-Ying, Cheng, Shih-Wei, Hsu, Yih-Chih
Format: Article
Language:English
Subjects:
Carcinoma, Hepatocellular - drug therapy
Carcinoma, Hepatocellular - radiotherapy
Caspase 3 - metabolism
Caspase 9 - metabolism
Cell Line, Tumor
Chlorophyll - analogs & derivatives
Chlorophyll - pharmacology
Collagen Type XI - metabolism
Cytochromes c - metabolism
Humans
Photochemotherapy
Photosensitizing Agents - pharmacology
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Summary:Photodynamic therapy (PDT) is an alternative anticancer treatment in which direct tumor-cell killing results from selective accumulation of photosensitizers in the tumor sites and phototoxicity occurs when light-activated photosensitizers transfer the energy to oxygen nearby to produce singlet oxygen. The objective of this study was to investigate the effects of PDT using chlorophyll derivatives such as pheophytin a (phe a), pheophytin b (phe b), pheophorbide a (pho a) and pheophorbide b (pho b) as the photosensitizers, and the 660 nm light-emitting diodes (LEDs) irradiation on human hepatocellular carcinoma cells (HuH-7). The drug concentration-dependent inhibition of HuH-7 cell viability was studied under LEDs irradiation (10 mW cm(-2)) at radiant exposure of 5.1 and 10.2 J cm(-2) by MTT assay. Significant inhibition of the survival of HuH-7 cells (
ISSN:1474-905X
1474-9092
DOI:10.1039/b704539e