Genotype of Giardia intestinalis isolates from children and dogs and its relationship to host origin

The presence of human Giardia in several animals suggests a zoonotic transmission. We studied G. Intestinalis isolates obtained from: children with diarrhea (n=6), asymptomatic children (n=7), axenic cultures (n=7) and dogs (n=11). The sequence corresponding to 16 S rRNA was amplified by PCR, sequen...

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Bibliographic Details
Published in:Parasitology research (1987) 2005-08, Vol.97 (1), p.1-6
Main Authors: ELIGIO-GARCIA, Leticia, CORTES-CAMPOS, Adrian, JIMENEZ-CARDOSO, Enedina
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Child
DNA, Protozoan - chemistry
DNA, Protozoan - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Dog Diseases - parasitology
Dogs
Fundamental and applied biological sciences. Psychology
General aspects
General aspects and techniques. Study of several systematic groups. Models
Genes, rRNA - genetics
Genotype
Giardia lamblia - classification
Giardia lamblia - genetics
Giardia lamblia - isolation & purification
Giardiasis - parasitology
Giardiasis - transmission
Giardiasis - veterinary
Humans
Invertebrates
Mammalia
Molecular Epidemiology
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
Sequence Alignment
Sequence Analysis, DNA
Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution
Zoonoses
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Summary:The presence of human Giardia in several animals suggests a zoonotic transmission. We studied G. Intestinalis isolates obtained from: children with diarrhea (n=6), asymptomatic children (n=7), axenic cultures (n=7) and dogs (n=11). The sequence corresponding to 16 S rRNA was amplified by PCR, sequenced and compared with genotypes A, B and Dog sequences reported in the Gene Bank database. Results show that 9/20 (45%) of children isolates belonged to genotype A and 11/20 (55%) showed some variable sites, allowing classification in three arbitrary clusters: A1, A2 and A3. In addition 7/11 (63%) of dog isolates were genotype A, including those dogs that lived in the same locality as the children lived, while 4/11 (37%) belonged to an arbitrary A4 cluster living in a different locality. In this study, genotype A was associated with samples from children and dogs, and, therefore, we could infer zoonotic transmission as a way of getting the disease.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-005-1368-9