A new mRNA splice variant coding for the human EP3-I receptor isoform

Abstract The cellular localization of prostaglandin E2 receptors (EP) and their corresponding transcripts were investigated in human gastric and vascular tissues. A strong staining of the EP3 receptor on the gastric glands, mucous cells, media of the mammary and pulmonary arteries was observed by im...

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Bibliographic Details
Published in:Prostaglandins, leukotrienes and essential fatty acids leukotrienes and essential fatty acids, 2007-10, Vol.77 (3), p.195-201
Main Authors: Kotelevets, L, Foudi, N, Louedec, L, Couvelard, A, Chastre, E, Norel, X
Format: Article
Language:English
Subjects:
Advanced Basic Science
Base Sequence
Biological and medical sciences
Endocrinology & Metabolism
Female
Fundamental and applied biological sciences. Psychology
Gastric Fundus - metabolism
Gastric Mucosa - metabolism
Humans
Immunohistochemistry
Male
Mammary Arteries - metabolism
Middle Aged
Molecular Sequence Data
Protein Isoforms - genetics
Pulmonary Artery - metabolism
Pulmonary Veins - metabolism
Receptors, Prostaglandin E - genetics
Receptors, Prostaglandin E, EP3 Subtype
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
Vertebrates: endocrinology
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Summary:Abstract The cellular localization of prostaglandin E2 receptors (EP) and their corresponding transcripts were investigated in human gastric and vascular tissues. A strong staining of the EP3 receptor on the gastric glands, mucous cells, media of the mammary and pulmonary arteries was observed by immunohistochemistry. We identified a new mRNA splice variant of the EP3 gene in human gastric fundic mucosa, mammary artery and pulmonary vessels. This EP3-Ic transcript contains exons 1, 2, 3, 5 and 6 of the EP3 gene and should be translated in the EP3-I isoform. In addition, the EP3-Ib , EP3-II , EP3-III , EP3-IV and EP3-e mRNAs were detected in these tissues.
ISSN:0952-3278
1532-2823
DOI:10.1016/j.plefa.2007.09.005