Transcript and protein level analyses of the interactions among PhoB, PhoR, PhoU and CreC in response to phosphate starvation in Escherichia coli

The responses to phosphate starvation by PhoR-PhoB two-component regulatory system are also affected by other regulatory systems in Escherichia coli. The interactions among PhoB, PhoR, PhoU and CreC were investigated at transcript and protein levels using real-time PCR and fluorescence resonance ene...

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Bibliographic Details
Published in:FEMS microbiology letters 2007-12, Vol.277 (2), p.254-259
Main Authors: Baek, Jong Hwan, Kang, Yeon Jae, Lee, Sang Yup
Format: Article
Language:English
Subjects:
Bacteria
Bacterial Proteins - metabolism
Bacteriology
Biological and medical sciences
E coli
Energy transfer
Escherichia coli
Escherichia coli - chemistry
Escherichia coli - genetics
Escherichia coli - metabolism
Escherichia coli Proteins - metabolism
Fluorescence
Fluorescence Resonance Energy Transfer
FRET
Fundamental and applied biological sciences. Psychology
Gene Expression Profiling
Gene Expression Regulation, Bacterial
Genetics
Membrane Transport Proteins - metabolism
Metabolism. Enzymes
Microbiology
Models, Biological
Pho regulon
Phosphate
Phosphate starvation response
Phosphates
Phosphates - metabolism
Protein Binding
Protein Kinases - metabolism
protein-protein interaction
Proteins
Reverse Transcriptase Polymerase Chain Reaction
Transcription
Transcription Factors - metabolism
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Summary:The responses to phosphate starvation by PhoR-PhoB two-component regulatory system are also affected by other regulatory systems in Escherichia coli. The interactions among PhoB, PhoR, PhoU and CreC were investigated at transcript and protein levels using real-time PCR and fluorescence resonance energy transfer analyses. CreC showed an interaction with PhoB, before an interaction between PhoB and PhoR, suggesting that this acts as a constant sensor for the early response to phosphate starvation. PhoU was found to interact with neither PhoB nor PhoR, while this acted as a phosphate starvation-inducible protein. These results showed the detailed time- and phosphate concentration-dependent interactions among PhoB, PhoR, PhoU and CreC in response to phosphate starvation in E. coli.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2007.00965.x