Homoglutathione confers tolerance to acifluorfen in transgenic tobacco [Nicotiana tabacum] plants expressing soybean [Glycine max] homoglutathione synthetase

Homoglutathione (hGSH), which is present in some leguminous plants, is preferred over GSH in in vitro conjugation of acifluorfen and fomesafen by glutathione S-transferase. To investigate the function of hGSH in in vivo detoxification of xenobiotics, we evaluated herbicide tolerance of transgenic to...

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Bibliographic Details
Published in:Plant and cell physiology 2005-08, Vol.46 (8), p.1428-1432
Main Authors: Sugiyama, A.(Kyoto Univ. (Japan)), Sekiya, J
Format: Article
Language:English
Subjects:
1-chloro-2
4-dinitrobenzene
acid-amino-acid ligases
ACIFLUORFEN
ACIFLUORFENE
Adaptation, Physiological - physiology
CDNB
chloroplasts
cytosol
enzyme activity
fomesafen
GLUTACION
GLUTATHION
GLUTATHIONE
Glutathione - analogs & derivatives
Glutathione - physiology
Glutathione S-transferase
glutathione synthetase
Glycine max
Glycine max - enzymology
GSHS
GST
HERBICIDAS
HERBICIDE
herbicide resistance
Herbicide tolerance
HERBICIDES
hGSH
hGSHS
Homoglutathione
homoglutathione synthase
homoglutathione synthetase
Nicotiana - drug effects
Nicotiana - enzymology
Nicotiana - genetics
Nicotiana - metabolism
Nicotiana tabacum
Nitrobenzoates - pharmacology
Peptide Synthases - genetics
plant physiology
plant proteins
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
Plants, Genetically Modified
Recombinant Proteins - genetics
root growth
seed germination
seedling growth
SOJA
SOYBEANS
TABAC
TABACO
TOBACCO
transgenes
TRANSGENIC PLANTS
Transgenic tobacco
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Summary:Homoglutathione (hGSH), which is present in some leguminous plants, is preferred over GSH in in vitro conjugation of acifluorfen and fomesafen by glutathione S-transferase. To investigate the function of hGSH in in vivo detoxification of xenobiotics, we evaluated herbicide tolerance of transgenic tobacco plants expressing soybean homoglutathione synthetase in the cytosol or chloroplasts. Transgenic plants synthesizing hGSH in the cytosol were more tolerant to acifluorfen than wild-type plants, whereas enhanced tolerance to fomesafen was not observed. Transgenic plants synthesizing hGSH in the chloroplasts showed no enhanced tolerance to acifluorfen or fomesafen.
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pci143