Sensitive bioassay of bupivacaine in human plasma by liquid-chromatography-ion trap mass spectrometry

A sensitive high-performance liquid chromatography–tandem mass spectrometric (HPLC–MS–MS) method, using an ion trap spectrometer, was developed for quantitation of bupivacaine in human plasma. Bupivacaine and an internal standard (ropivacaine) were extracted in a single step from 100 μL of alkaliniz...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2005-09, Vol.39 (3), p.587-592
Main Authors: Hoizey, Guillaume, Lamiable, Denis, Robinet, Arnaud, Ludot, Hugues, Malinovsky, Jean-Marc, Kaltenbach, Matthieu L., Binet, Laurent, Boulanger, Christian, Millart, Hervé
Format: Article
Language:English
Subjects:
Acetonitriles - chemistry
Amides - analysis
Amides - pharmacokinetics
Anaesthetics
Analysis
Analytical, structural and metabolic biochemistry
Anesthetics, Local - analysis
Anesthetics, Local - chemistry
Biological and medical sciences
Biological Assay - methods
Bupivacaine
Bupivacaine - analysis
Bupivacaine - chemistry
Bupivacaine - pharmacokinetics
Calibration
Chemistry, Pharmaceutical - methods
Chromatography, Liquid - methods
Drug Industry - methods
Formates - chemistry
Fundamental and applied biological sciences. Psychology
General pharmacology
Humans
Liquid chromatography-mass spectrometry
Mass Spectrometry - methods
Medical sciences
Pharmacology. Drug treatments
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization
Temperature
Time Factors
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Summary:A sensitive high-performance liquid chromatography–tandem mass spectrometric (HPLC–MS–MS) method, using an ion trap spectrometer, was developed for quantitation of bupivacaine in human plasma. Bupivacaine and an internal standard (ropivacaine) were extracted in a single step from 100 μL of alkalinized plasma with diethyl-ether. The mobile phase consisted of acetonitrile with 0.1% formic acid (50:50, v/v), and was delivered at a flow rate of 0.3 mL/min. The effluent was detected by MS–MS in positive ion mode. Ionisation was performed, using an electrospray ion source, operating at 200 °C. The selected reaction monitoring transitions m/ z 289 → m/ z 140 and m/ z 275 → m/ z 126 were chosen for bupivacaine and ropivacaine, respectively. Calibration curves were linear over the concentration range of 3.90–500 μg/L with determination coefficients >0.996. The method is accurate (bias
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2005.03.042