Comparative gene and protein expression in primary cultures of epithelial cells from benign prostatic hyperplasia and prostate cancer

Primary cultures are widely used to investigate the disease-specific biology of prostate cancer and benign prostatic hyperplasia (BPH). To identify genes differentially expressed between epithelial cells cultured from adenocarcinomas versus BPH tissues, we used probe array technology. Gene expressio...

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Bibliographic Details
Published in:Cancer letters 2005-09, Vol.227 (2), p.213-222
Main Authors: Rose, Amy, Xu, Yue, Chen, Zuxiong, Fan, Zengbin, Stamey, Thomas A., McNeal, John E., Caldwell, Mitchell, Peehl, Donna M.
Format: Article
Language:English
Subjects:
Adenocarcinoma - genetics
Adenocarcinoma - metabolism
Adenocarcinoma - pathology
Benign prostatic hyperplasia
Biomarkers, Tumor - metabolism
Cell culture
Cell Proliferation
Cytokeratin 16
Deoxyribonucleic acid
DNA
Epithelial Cells - metabolism
Epithelial Cells - pathology
Gene expression
Gene Expression Profiling
Humans
Immunoblotting
Kinases
Male
Oligonucleotide Array Sequence Analysis
Prostate
Prostate cancer
Prostatic Hyperplasia - genetics
Prostatic Hyperplasia - metabolism
Prostatic Hyperplasia - pathology
Prostatic Neoplasms - genetics
Prostatic Neoplasms - metabolism
Prostatic Neoplasms - pathology
Protein expression
Proteins
Reverse Transcriptase Polymerase Chain Reaction
RNA polymerase
Tissue Culture Techniques
Tumor Cells, Cultured
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Summary:Primary cultures are widely used to investigate the disease-specific biology of prostate cancer and benign prostatic hyperplasia (BPH). To identify genes differentially expressed between epithelial cells cultured from adenocarcinomas versus BPH tissues, we used probe array technology. Gene expression profiles were evaluated on Affymetrix Human Cancer G110 Array Chips containing ∼1900 cancer-related genes. After defined statistical analysis, genes that were over-expressed in cancer cultures were identified. Protein expression of four of the differentially expressed genes was measured in immunoblots, and the expression of two other genes was measured by real-time reverse transcription-polymerase chain reaction (RT-PCR). While no gene or protein was consistently over-expressed in all cancer versus BPH cell cultures, cytokeratin 16 protein was highly elevated in several of the cancer cultures, suggesting that a hyperproliferative phenotype may be characteristic of prostate cancer cells.
ISSN:0304-3835
1872-7980
DOI:10.1016/j.canlet.2005.01.037