Planarity and Constraint of the Carbonyl Groups in 1,2-Diones Are Determinants for Selective Inhibition of Human Carboxylesterase 1

Carboxylesterases (CE) are ubiquitous enzymes responsible for the detoxification of xenobiotics, including numerous clinically used drugs. Therefore, the selective inhibition of these proteins may prove useful in modulating drug half-life and bioavailability. Recently, we identified 1,2-diones as po...

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Bibliographic Details
Published in:Journal of medicinal chemistry 2007-11, Vol.50 (23), p.5727-5734
Main Authors: Hyatt, Janice L, Wadkins, Randy M, Tsurkan, Lyudmila, Hicks, Latorya D, Hatfield, M. Jason, Edwards, Carol C, Ross, Charles R, Cantalupo, Stephanie A, Crundwell, Guy, Danks, Mary K, Guy, R. Kip, Potter, Philip M
Format: Article
Language:English
Subjects:
Acetylcholinesterase - chemistry
Biological and medical sciences
Butyrylcholinesterase - chemistry
Carboxylic Ester Hydrolases - antagonists & inhibitors
Carboxylic Ester Hydrolases - chemistry
Crystallography, X-Ray
Glyoxal - analogs & derivatives
Glyoxal - chemical synthesis
Glyoxal - chemistry
Humans
Intestines - enzymology
Medical sciences
Miscellaneous
Models, Molecular
Molecular Structure
Pharmacology. Drug treatments
Quantitative Structure-Activity Relationship
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Summary:Carboxylesterases (CE) are ubiquitous enzymes responsible for the detoxification of xenobiotics, including numerous clinically used drugs. Therefore, the selective inhibition of these proteins may prove useful in modulating drug half-life and bioavailability. Recently, we identified 1,2-diones as potent inhibitors of CEs, although little selectivity was observed in the inhibition of either human liver CE (hCE1) or human intestinal CE (hiCE). In this paper, we have further examined the inhibitory properties of ethane-1,2-diones toward these proteins and determined that, when the carbonyl oxygen atoms are cis-coplanar, the compounds demonstrate specificity for hCE1. Conversely, when the dione oxygen atoms are not planar (or are trans-coplanar), the compounds are more potent at hiCE inhibition. These properties have been validated in over 40 1,2-diones that demonstrate inhibitory activity toward at least one of these enzymes. Statistical analysis of the results confirms the correlation (P < 0.001) between the dione dihedral angle and the preferential inhibition of either hiCE or hCE1. Overall, the results presented here define the parameters necessary for small molecule inhibition of human CEs.
ISSN:0022-2623
1520-4804
DOI:10.1021/jm0706867