A HPLC method for the analysis of germacrone in rabbit plasma and its application to a pharmacokinetic study of germacrone after administration of zedoary turmeric oil

A validated new, simple and highly sensitive reversed-phase HPLC method is developed for studying the pharmacokinetics of germacrone after intravenous administration of zedoary turmeric oil (ZTO) oil-in-water microemulsion. The method did not require a complex and expensive equipment. A high extract...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2005-09, Vol.823 (2), p.172-176
Main Authors: You, Jian, Cui, Fu-de, Li, Qing-po, Yong-sheng, Wang, Han, Xu, Yu, Ying-wei
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Animals
Area Under Curve
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Curcuma - chemistry
Dose-Response Relationship, Drug
Drugs, Chinese Herbal - pharmacokinetics
Fundamental and applied biological sciences. Psychology
General pharmacology
Germacrone
HPLC
Injections, Intravenous
Male
Medical sciences
Metabolic Clearance Rate
Pharmacokinetic
Pharmacology. Drug treatments
Plant Oils - administration & dosage
Protein precipitation
Rabbits
Reproducibility of Results
Sesquiterpenes, Germacrane - blood
Sesquiterpenes, Germacrane - pharmacokinetics
Solvent extraction
Zedoary turmeric oil
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Summary:A validated new, simple and highly sensitive reversed-phase HPLC method is developed for studying the pharmacokinetics of germacrone after intravenous administration of zedoary turmeric oil (ZTO) oil-in-water microemulsion. The method did not require a complex and expensive equipment. A high extraction recovery (>80%) of germacrone was obtained. Linear calibration curves obtained with the peak-area ratio ( y) of germacrone to internal standard (tanshinoneIIA) versus drug concentration ( x) were found to be linear between 8.08 and 808 ng/ml. The limit of quantitation was 8.08 ng/ml.The monitored compounds were completely separated from others in ZTO and from endogenous species in plasma by HPLC. Pharmacokinetic investigations were performed on 18 male rabbits after intravenous administration of ZTO microemulsion via the ear vein at germacrone doses of 3.2, 6.4 and 9.6 mg/kg. The plasma concentration–time data fit to a two-compartment intravenous model with a weight of 1/ C 2 ( C: germacrone concentration in plasma). Germacrone exhibited linear pharmacokinetics after intravenous administration of ZTO microemulsion to rabbits over the germacrone dose range 3.2–9.6 mg/ml.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2005.06.032