Focal gene misexpression in zebrafish embryos induced by local heat shock using a modified soldering iron

Misexpression of genes in a temporally and spatially controlled fashion is an important tool for assessing gene function during development. Because few tissue‐specific promoters have been identified in zebrafish, inducible systems such as the Cre/LoxP and Tet repressor systems are of limited utilit...

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Bibliographic Details
Published in:Developmental dynamics 2007-11, Vol.236 (11), p.3071-3076
Main Authors: Hardy, Melissa E., Ross, Louis V., Chien, Chi‐Bin
Format: Article
Language:English
Subjects:
Animals
Animals, Genetically Modified
conditional expression
Danio rerio
Embryo, Nonmammalian - metabolism
Gene Expression Regulation, Developmental
Heat-Shock Response
Hot Temperature
HSP70 Heat-Shock Proteins - genetics
hsp70, GFP
Intercellular Signaling Peptides and Proteins - genetics
Nerve Tissue Proteins - genetics
transgene
Zebrafish - embryology
Zebrafish - genetics
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Summary:Misexpression of genes in a temporally and spatially controlled fashion is an important tool for assessing gene function during development. Because few tissue‐specific promoters have been identified in zebrafish, inducible systems such as the Cre/LoxP and Tet repressor systems are of limited utility. Here we describe a new method of misexpression: local heat shock using a modified soldering iron. Zebrafish carrying transgenes under the control of a heat shock promoter (hsp70) are focally heated with the soldering iron to induce gene expression in a small area of the embryo. We have validated this method in three stable transgenic lines and at three developmental timepoints. Local heat shock is a fast, easy, and inexpensive method for gene misexpression. Developmental Dynamics 236:3071–3076, 2007. © 2007 Wiley‐Liss, Inc.
ISSN:1058-8388
1097-0177
DOI:10.1002/dvdy.21318