Prognostic significance of intraperitoneal cancer cells in gastric carcinoma: detection of cytokeratin 20 mRNA in peritoneal washes, in addition to detection of carcinoembryonic antigen

In patients with gastric cancer, the presence of gastric cancer cells in the peritoneal cavity detected by cytologic examination, is a significant prognostic factor. A more sensitive, reverse transcriptase-polymerase chain reaction (RT-PCR) technique, amplifying carcinoembryonic antigen (CEA), was i...

Full description

Saved in:
Bibliographic Details
Published in:Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 2005-08, Vol.8 (3), p.142-148
Main Authors: Kodera, Yasuhiro, Nakanishi, Hayao, Ito, Seiji, Yamamura, Yoshitaka, Fujiwara, Michitaka, Koike, Masahiko, Hibi, Kenji, Ito, Katsuki, Tatematsu, Masae, Nakao, Akimasa
Format: Article
Language:English
Subjects:
Biomarkers, Tumor - genetics
Biomarkers, Tumor - metabolism
Carcinoembryonic Antigen - genetics
Carcinoembryonic Antigen - metabolism
Gastric cancer
Humans
Intermediate Filament Proteins - genetics
Intermediate Filament Proteins - metabolism
Keratin-20
Lymphatic Metastasis - diagnosis
Neoplasm Invasiveness - diagnosis
Neoplasm Staging
Oncology
Peritoneal Lavage
Peritoneal Neoplasms - genetics
Peritoneal Neoplasms - metabolism
Peritoneal Neoplasms - secondary
Prognosis
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
RNA, Neoplasm - genetics
RNA, Neoplasm - metabolism
Sensitivity and Specificity
Stomach Neoplasms - genetics
Stomach Neoplasms - metabolism
Survival Rate
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In patients with gastric cancer, the presence of gastric cancer cells in the peritoneal cavity detected by cytologic examination, is a significant prognostic factor. A more sensitive, reverse transcriptase-polymerase chain reaction (RT-PCR) technique, amplifying carcinoembryonic antigen (CEA), was introduced as a new detection system, but produced some false-positive results. A search for other molecular markers is ongoing. Peritoneal washes were obtained from 195 patients with gastric carcinoma during surgery. Cytokeratin 20 (CK20) mRNA levels were quantified, in addition to those of CEA, using the LightCycler, and the feasibility of CK20 as a target was evaluated. CK20 was limited, in terms of sensitivity, for detecting disseminated cancer cells (sensitivity, 63%; specificity, 91%; positive predictive value, 70%; and negative predictive value, 88%). Multimarker analysis was performed, in which samples positive for either CK20 or CEA mRNA were considered to be positive for cancer cells. Multivariate analysis identified the multimarker analysis as a significant independent prognostic determinant. CK20 RT-PCR produced information that could add a significant impact to the knowledge obtained by CEA RT-PCR, although detection by CK20 alone was not sufficiently sensitive to replace CEA in the detection system.
ISSN:1436-3291
1436-3305
DOI:10.1007/s10120-005-0318-7