Combinatorial screening of cell proliferation on poly( l-lactic acid)/poly( d,l-lactic acid) blends

We have combined automated fluorescence microscopy with a combinatorial approach for creating polymer blend gradients to yield a rapid screening method for characterizing cell proliferation on polymer blends. A gradient in polymer blend composition of poly( l-lactic acid) (PLLA) and poly( d,l-lactic...

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Bibliographic Details
Published in:Biomaterials 2005-12, Vol.26 (34), p.6906-6915
Main Authors: Simon, Carl G., Eidelman, Naomi, Kennedy, Scott B., Sehgal, Amit, Khatri, Chetan A., Washburn, Newell R.
Format: Article
Language:English
Subjects:
3T3 Cells
Animals
Artificial Intelligence
Biocompatible Materials - chemistry
Cell Count - methods
Cell Proliferation
Combinatorial Chemistry Techniques
Combinatorial methods
Fibroblasts - cytology
Fibroblasts - physiology
Image Interpretation, Computer-Assisted - methods
Lactic Acid - analysis
Lactic Acid - chemistry
Materials Testing - methods
Mice
Microscopy, Fluorescence - methods
Microspectroscopic FTIR
Osteoblast
Polyesters
Polylactic acid
Polymer blends
Polymers - analysis
Polymers - chemistry
Spectroscopy, Fourier Transform Infrared - methods
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Summary:We have combined automated fluorescence microscopy with a combinatorial approach for creating polymer blend gradients to yield a rapid screening method for characterizing cell proliferation on polymer blends. A gradient in polymer blend composition of poly( l-lactic acid) (PLLA) and poly( d,l-lactic acid) (PDLLA) was created in the form of a strip-shaped film and was annealed to allow PLLA to crystallize. Fourier transform infrared (FTIR) microspectroscopy was used to determine the composition in the gradients and atomic force microscopy was used to characterize surface topography. Osteoblasts were cultured on the gradients and proliferation was assessed by automated counting of cells using fluorescence microscopy. Surface roughness varied with composition, was smooth on PDLLA-rich regions and was rough on the PLLA-rich regions. Cell adhesion was similar on all regions of the gradients while proliferation was faster on the smooth, PDLLA-rich end of the gradients than on the rough, PLLA-rich end of the gradients. These results demonstrate the feasibility of a new, combinatorial approach for evaluating cell proliferation on polymer blends.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2005.04.050