A standard protocol for obtaining fish chromosomes under post-mortem conditions

In order to improve cytogenetical analyses on fish, especially focusing on delicate and rare species, we have adopted a new in vitro methodology using dead animals. The results obtained from 24 neotropical freshwater and marine fish species demonstrate that chromosomes can be obtained under post-mor...

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Bibliographic Details
Published in:Micron (Oxford, England : 1993) England : 1993), 2007-01, Vol.38 (3), p.214-217
Main Authors: de Cáscia Barreto Netto, Maria Rita, Pauls, Erica, de Mello Affonso, Paulo Roberto A.
Format: Article
Language:English
Subjects:
Animals
Chromosomes
Cytogenetic Analysis - methods
Cytogenetic Analysis - standards
Fishes - genetics
Freshwater fish
In vitro cytogenetical technique
Marine fish
Microscopy
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Summary:In order to improve cytogenetical analyses on fish, especially focusing on delicate and rare species, we have adopted a new in vitro methodology using dead animals. The results obtained from 24 neotropical freshwater and marine fish species demonstrate that chromosomes can be obtained under post-mortem conditions. Significantly, the samples analyzed provided reliable cytogenetical data in nearly all cases. Other advantages of this new methodology are also discussed.
ISSN:0968-4328
1878-4291
DOI:10.1016/j.micron.2006.07.019