Absorption microspectroscopy, theory and applications in the case of the photosynthetic compartment

We performed microspectroscopic evaluation of the pigment composition of the photosynthetic compartments of both algae and higher plants. The feasibility of microspectroscopy for discriminating among species and/or phylogenetic groups was tested on laboratory cultures. Gaussian bands decompositions,...

Full description

Saved in:
Bibliographic Details
Published in:Micron (Oxford, England : 1993) England : 1993), 2007-01, Vol.38 (3), p.197-213
Main Authors: Barsanti, Laura, Evangelista, Valtere, Frassanito, Anna Maria, Vesentini, Nicoletta, Passarelli, Vincenzo, Gualtieri, Paolo
Format: Article
Language:English
Subjects:
Algae
Biological Evolution
Carotenoids - analysis
Chlorophyll - analysis
Endosymbiosis
Eukaryota - chemistry
Eukaryota - classification
Fourth-derivative transformation
Gaussian decomposition
Image Processing, Computer-Assisted - methods
Microscopy - methods
Microspectroscopy
Organelles - chemistry
Phycobiliproteins - analysis
Pigment
Pigments, Biological - analysis
Plants - chemistry
Spectrum Analysis - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We performed microspectroscopic evaluation of the pigment composition of the photosynthetic compartments of both algae and higher plants. The feasibility of microspectroscopy for discriminating among species and/or phylogenetic groups was tested on laboratory cultures. Gaussian bands decompositions, and a fitting algorithm, together with fourth-derivative transformation of absorbance spectra, provided a reliable discrimination among chlorophylls, phycobiliproteins and carotenoids. Comparative analysis of absorption spectra highlighted the evolutionary grouping of the algae into three main lineages in accordance with the most recent endosymbiotic theories.
ISSN:0968-4328
1878-4291
DOI:10.1016/j.micron.2006.07.015