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Prostacyclin receptor signaling and early embryo development in the mouse
BACKGROUND Prostacyclin (PGI2) plays an important role in mouse embryo development and implantation. However, it is unclear whether its action is mediated via the I prostaglandin receptor (IP). METHODS We compared the preimplantation development of IP deleted (IP−/−) embryos and wild-type (WT) embry...
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Published in: | Human reproduction (Oxford) 2007-11, Vol.22 (11), p.2851-2856 |
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Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that cite this one |
Online Access: | Get full text |
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Summary: | BACKGROUND Prostacyclin (PGI2) plays an important role in mouse embryo development and implantation. However, it is unclear whether its action is mediated via the I prostaglandin receptor (IP). METHODS We compared the preimplantation development of IP deleted (IP−/−) embryos and wild-type (WT) embryos. We also evaluated the effect of iloprost, a stable PGI2 analog, and L-165041, a peroxisome proliferator activated receptor δ (PPARδ) ligand, on IP−/− versus WT embryos. Finally, we compared the development of heterozygous IP deficient embryos carrying a normal maternal IP allele versus paternal IP allele. RESULTS Development of IP−/− embryos lagged behind WT embryos and was not enhanced by either the PGI2 analog or the PPARδ ligand. WT embryos had slightly higher, although statistically not significant, implantation rates than IP−/− embryos. Heterozygous IP deficient embryos carrying a normal maternal IP allele showed better development and responded to the PGI2 analog, unlike those carrying the normal paternal IP allele. CONCLUSIONS IP receptors play an important role in preimplantation embryo development and mediate the embryo's response to exogenous PGI2. Early embryo development depends on the oocyte IP receptor. |
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ISSN: | 0268-1161 1460-2350 |
DOI: | 10.1093/humrep/dem304 |